Basic Information

Insect: Megachile rotundata
Gene Symbol: MBD-R2
Assembly: GCA_000220905.1
Location: NW:772377-782044[-]

Transcription Factor Domain

TF Family: MBD
Domain: MBD domain
PFAM: PF01429
TF Group: Unclassified Structure
Description: The Methyl-CpG binding domain (MBD) binds to DNA that contains one or more symmetrically methylated CpGs [2]. DNA methylation in animals is associated with alterations in chromatin structure and silencing of gene expression. MBD has negligible non-specific affinity for DNA. In vitro foot-printing with MeCP2 showed the MBD can protect a 12 nucleotide region surrounding a methyl CpG pair [2]. MBDs are found in several Methyl-CpG binding proteins and also DNA demethylase [1].
Hmmscan Out: # of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc

1 3 0.61 1.5e+03 -2.0 0.1 44 60 406 422 403 440 0.79

2 3 2.3 5.6e+03 -3.8 0.0 40 50 446 456 445 466 0.82

3 3 9.5e-18 2.4e-14 51.9 0.2 6 69 546 609 541 617 0.91

#	of	c-Evalue	i-Evalue	score	bias	hmm coord from	hmm coord to	ali coord from	ali coord to	env coord from	env coord to	acc
1	3	0.61	1.5e+03	-2.0	0.1	44	60	406	422	403	440	0.79
2	3	2.3	5.6e+03	-3.8	0.0	40	50	446	456	445	466	0.82
3	3	9.5e-18	2.4e-14	51.9	0.2	6	69	546	609	541	617	0.91

Sequence Information

Coding Sequence: ATGAAGCGGCGGCGTATAGCGCGGTCGCGTGGTAAAGGGATACCGCCTTTGAGGATGATGAAAATGGCGCGCAAATGTTGCGTGCGTAGCTGTGAGGTTGATGTGCAAGATGCTCGAGCCAAGGGTTTACCGCTTCATAGGTTTCCAAAAGACAGCAATTTGAGAAGTAAATGGTTAGCCAGTGGTGGATTCGAGACAACTTTTAAACCTTCACCGGGTCAGGTTGTCTGCCACAGACATTTTAAACGTGCTGATTACGAAGCCGCTAAGGGACATAAATTACTTCTGCGTAAAGGCAGTGTTCCGTCAGTTTTTGCAGATTATGACAATCATCCAGATCCTGTGATAATGTCTGTGAAATCATCAACTTCTTATGCGCAGGAGGATTTGGATCTCATTAATTCtgagattttaaatttagagcAATCTATTTCACCATTGGTATCTGAAGCAAAAACACAAAAGGTTGATACCTCTAAAGAAACATGTGCTTCTGAACCAGCATCATTAGTTGATTCTTCTAATGAGTTAGATTCCAAAGAAATACTTGATAatggatataaaaattcgaatgtaaaagaaaaaaacagcataaaacaagaaaaagttCCTGCACAGCAggaagtaaatgaaaattcagaagtaCAAGTAAATACTGTTGCAGTGCAGTTAGGAATTGTAGAATCAGATATAGCAATGAAGGATATACAAAAAAGTTTGATCATAaaggaagaattaaaaaacgtTAAAATGGAGGATGAAAAAGTATTTGAGAAAACAGATGAATTAAAACCAAAGATTTTGAACCGAGgtggattaaaattttttcctGGTGCCAAATTAGAAGCAAaagatttcaatgaaaaatgGTATTCAGCTAAAGTAGTAGAAACTGATTGGGATGAAAGAGAAGTCTTGATTCATTTTGATAAATGGAGCTCAAGATTTGACGAATGGATACCTATGGATAGCTCCAGATTACGAGTATTACAAACACAATCAAAtGAAGAAACTTGGGATCTGCCATCTTCggaagaaaaaatgaaagatttttCAGTAGGAGAAAGGATACTTGCTACATGGGCTGATGGTAGAAAATATCCAGCCAAAGTGAATGCAGTTTTAGGGAATGATAGGTATGATGTACTCTTTGATGATGGATATGCAAAGACAGTTAAATCgtcaaaaatgacaaaaattactCCAACATCCACAAAGCAATTTAATCAAACTGAAGAATATATAGGAAGCAAACAAGAAAGAAGagataagaaaagaaaacataCAGTCATGGAGTTATTTCATACACATTCTagaaaacgaacaaagaatgAAGGagataaattatcaaaaaaacAAGAGGTAGCAATACATGAAAATGGAGATGTACcagaaaaaattgatttagatGGTACTTTGTTTGGTCCTTGTTATGATCCCGGAACGGATCTATTACGAGGATTTGACACtaatatatcaaaaataaaaagttttccAAAAAAGACTAAGAAAGATATCCCAAGAACTGATATAAGTCAGGAAGATGATGTAGGACCTGAGTGGATAGATGGAGAACCACAAGGAACTGAATCTTATATAGTAGATGGAAATGATGGGCCTCGTCGCTCAATAATCGTTGCAGATAAAAGATTACCGCCTGGATGGCAAAAACATTTTACGCAAAGAAAAGCCGGTACATCTGCTGGAAAATGGGATGTCTTATTTCTTCATAAATCAAGTGGAAAAAAGTTTAGATCGCGAAATGATATCAAAGTATTTCTGGAAAACCAAGGGCAGTTTGATTTTGAtcctgaaaaatttgatttttgtattcatcggaaaaagaaaaactatggacaaaaagTAAAACAACCTATTACTATGGATGTACCAAAAAAGATTAAAACTTTATTACCTAAAGCAAAAGCACCAACATCAGAAGATTCGTTGCTGGTTTCAACAAGTACACCAGTTACAACATTAGTATCTACATCAACAACATCCATCACAGATGGTGCTGTTTTCATTGGTGGACTTCGCGTAGAAATGGAAGACAGTGCTTACAAGTGTCCAAAACAGGgatgtaataaaacatttcgTAAAGAGAATCTTTTACAAATGCATATAAAACATTATCATCCagaatattccaaatttttgggATCTACGCCAAATGTTGCAGATTTAGCATACGCAAGAACAATTGGGGAATCCGTTGAAGATATTATTCcgaaaaaatcaaataatttactaGACAAATGTAATAAACTTGGAAAGAAAAAACCTGTTCATGAGAAAACATTATACACTGCACCATCATCTCTAACGACTTTTGTTCAATCTACATCAccaaaagtaattttatcaaCGGCATCAGACATAGAAGATGAAATAGATCAAGTAGAAAAAAGTAACGATGTACCAGCAGAAGACATTAAATCAGAAAGAATGTCTCCAATTTCGAGTTATAGCGTCGATATGGatgatgaaattgaaaagaaacgaGATGCTTCAGCAATGTCCCCTGGAACATTATTTGACATGAAAATCAAAGAAGAAAGAACACAAACTGGCATTAAAACTCTTCTTCCTGTAAGACCGGCAGTATCTTCAGAGATACAAAGAATTGATAGACCAAAATCTTTAGACgaagttattaatattgatagaATGAAGGGTCAACGAAAACGACAATTATCAGAATACAGTTCTGATGGATTAAATAGGAATAAACGACGTCAAGGTATTCAGGAACTAGATGAATATGGTGATTTAGATGATAGTGCTTTGGATACTGATGAACCGACGACGCTTACATATAGATATAGTCGCAGAAAATCAGATTCCAGAAGTGATGAAAATAGCCAGAGTAgTCAACTAAATGATTCTCGCGTTGAAAAAGATGATACTTTGAAAGGGGATATTGCTAAAAAAGATACTAATGATGGGGAagAAACTGAAGGTGTTATGATGATGATTAATGGAGAAATGATAAAGGTTGAACAACTGCGTagagaagaaataataaattgtacctGTGGATTTATGGAAGAAGATGGTTTAATGATACAGTGCGACCTTTGTTTATGTTGGCAACACGGGCATTGTAACGCaatagaaagagaaaaagatgTACCCGAAAAAtatgtttgttatatttgtcAGAATCCATATCGACAACGGCCATCTAAAAAGTATTTCCATGATCAAGATTGGGTGAAGGAGGGAAAACTGCCAATGTTATCTGATCGAACCAAAAATCAGTATCGAATAAATAAGAGGACGGCTATGTTGAAACGTTCTTATGATTTAGTCGCATCCCTCTTACAAATACAGCAAGTTTTACATAGTTTAAgagtaaaaattaatgtagcTCAaaaaaaggATCATCCAAAATTGTACCTTTGGGcaaaaaattgggaaaaagtGGAAGTACCGAAACCAGATATAACACCAGTACCAATAATGGAAGTTACCAAATCAGGAAATGATGATTCAGATAGTACAATTGAACCGTCTCGTCGAGTTGAAGTGAAGACAGAAATAAAGTTTACTTTAAAGGATAATCatgatgaaaaattaatagctTCAGATTCAGAGttgatgaaaattttagagGAAGATAATCCAACTTCGGACGATTCCAAAGTTTCGTGTAAGAAAGAAGATCtgacaaatgaaaataaaagtcaTATTCTTCTTGATGCACTCACGAAAAGTGCTGCTGCTgaagaaaagtataaaaattcagcGACTTCGGAAATAAAAACGGATACagatttattaaataatcaaaatattgtaTCAGAAAATGATATACCTATACCAGCTGCTTCAGAAAACAGTATGCATGAGGAATTGAATAAACATGAAACATCGACGCCGATACAGCCTTTTATTCCACAACCAGAAGCACCAATTGATCCAGGGGAGTGCCGAATGCGATTGTTAGAAcatattgaacattttcaaaatcatataGACGCAAGATTAACATTTATTGAGGCACAAGTTTGTGCATTAGAAGCTATGGATCCTGAAGATGTATCATCTAGTCCAGATATTCAACCACGAACAAAACAAACAGTTCAAATGCTGCTTCGAGATTTAAATACAGTGCGAAAGTTAGCTGCtctatgttaa
Protein Sequence: MKRRRIARSRGKGIPPLRMMKMARKCCVRSCEVDVQDARAKGLPLHRFPKDSNLRSKWLASGGFETTFKPSPGQVVCHRHFKRADYEAAKGHKLLLRKGSVPSVFADYDNHPDPVIMSVKSSTSYAQEDLDLINSEILNLEQSISPLVSEAKTQKVDTSKETCASEPASLVDSSNELDSKEILDNGYKNSNVKEKNSIKQEKVPAQQEVNENSEVQVNTVAVQLGIVESDIAMKDIQKSLIIKEELKNVKMEDEKVFEKTDELKPKILNRGGLKFFPGAKLEAKDFNEKWYSAKVVETDWDEREVLIHFDKWSSRFDEWIPMDSSRLRVLQTQSNEETWDLPSSEEKMKDFSVGERILATWADGRKYPAKVNAVLGNDRYDVLFDDGYAKTVKSSKMTKITPTSTKQFNQTEEYIGSKQERRDKKRKHTVMELFHTHSRKRTKNEGDKLSKKQEVAIHENGDVPEKIDLDGTLFGPCYDPGTDLLRGFDTNISKIKSFPKKTKKDIPRTDISQEDDVGPEWIDGEPQGTESYIVDGNDGPRRSIIVADKRLPPGWQKHFTQRKAGTSAGKWDVLFLHKSSGKKFRSRNDIKVFLENQGQFDFDPEKFDFCIHRKKKNYGQKVKQPITMDVPKKIKTLLPKAKAPTSEDSLLVSTSTPVTTLVSTSTTSITDGAVFIGGLRVEMEDSAYKCPKQGCNKTFRKENLLQMHIKHYHPEYSKFLGSTPNVADLAYARTIGESVEDIIPKKSNNLLDKCNKLGKKKPVHEKTLYTAPSSLTTFVQSTSPKVILSTASDIEDEIDQVEKSNDVPAEDIKSERMSPISSYSVDMDDEIEKKRDASAMSPGTLFDMKIKEERTQTGIKTLLPVRPAVSSEIQRIDRPKSLDEVINIDRMKGQRKRQLSEYSSDGLNRNKRRQGIQELDEYGDLDDSALDTDEPTTLTYRYSRRKSDSRSDENSQSSQLNDSRVEKDDTLKGDIAKKDTNDGEETEGVMMMINGEMIKVEQLRREEIINCTCGFMEEDGLMIQCDLCLCWQHGHCNAIEREKDVPEKYVCYICQNPYRQRPSKKYFHDQDWVKEGKLPMLSDRTKNQYRINKRTAMLKRSYDLVASLLQIQQVLHSLRVKINVAQKKDHPKLYLWAKNWEKVEVPKPDITPVPIMEVTKSGNDDSDSTIEPSRRVEVKTEIKFTLKDNHDEKLIASDSELMKILEEDNPTSDDSKVSCKKEDLTNENKSHILLDALTKSAAAEEKYKNSATSEIKTDTDLLNNQNIVSENDIPIPAASENSMHEELNKHETSTPIQPFIPQPEAPIDPGECRMRLLEHIEHFQNHIDARLTFIEAQVCALEAMDPEDVSSSPDIQPRTKQTVQMLLRDLNTVRKLAALC

Similar Transcription Factors

Sequence clustering based on sequence similarity using MMseqs2

100% Identity: iTF_00964525; iTF_00962510; iTF_00961875; iTF_00963188; iTF_00963892; iTF_00964474; iTF_00961823; iTF_00962559; iTF_01122433; iTF_01123107; iTF_01122484; iTF_01123061;
90% Identity: iTF_01122433;
80% Identity: iTF_00963892;