Basic Information

Transcription Factor Domain

TF Family: PAX
Domain: PAX domain
PFAM: PF00292
TF Group: Helix-turn-helix
Description: The paired domain, a ~126 amino acid DNA-binding domain, is found in eukaryotic transcription regulatory proteins involved in embryogenesis. Initially identified in Drosophila’s paired (prd) protein, it typically resides in the N-terminal region and may be followed by an octapeptide, a homeodomain, or a Pro-Ser-Thr-rich C terminus. Paired domain proteins act as transcription repressors or activators, with DNA-binding specificity mediated by three subdomains. Crystal structures reveal a bipartite DNA-binding paired domain: an N-terminal subdomain (PAI) and a C-terminal subdomain (RED), linked by a flexible linker. Both subdomains contain a helix-turn-helix motif that binds DNA's major groove, while the linker may bind the minor groove. Variations in domain usage across Pax proteins and isoforms determine sequence specificity.
Hmmscan Out: # of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc

1 2 1.1e-11 1.5e-08 35.8 1.5 19 103 7 90 2 97 0.90

2 2 0.54 7e+02 1.3 0.1 40 58 93 111 90 147 0.79

#	of	c-Evalue	i-Evalue	score	bias	hmm coord from	hmm coord to	ali coord from	ali coord to	env coord from	env coord to	acc
1	2	1.1e-11	1.5e-08	35.8	1.5	19	103	7	90	2	97	0.90
2	2	0.54	7e+02	1.3	0.1	40	58	93	111	90	147	0.79

Coding Sequence: atGGGAAGATTTtctgtaaataataatattctctggcaaATTATTGCCTTATATAAAGACAAAAACAGCCAGAGAGAAATTGTTAGGAAATTAGAAATTAGCAAAACATGTGTGCAGCACACAATAAAAAGATTTGTCGAAACAGGGTCTGTAAAGCACAAAACTCGAACTGGACGTCCAAAAAGGGTAACAGACAGAGTTAAATCAAAGTTAATACAGATTGTCAGGAAAAACAGATCAATGACTAGTCGAGAAATTGCAAATGAGTTGGAATACTCAACACAAGTGAAAATTTCAGCCGCACACATTCGAAAAATTCTTTGCAAAGTAAATTATAAAAGTCGTACAGCTGCAAAAAAACCTTTCATTACTCctaaaaatgcaataaatcGCAAGCGGTGGTGTAGAGAACGTAAGAATTGGACTGTACAACAGTGGAAAAAGATTGTATTTTCTGATGAAAGTACGTTTCAACTTTTTCCAAACAGTAGAAGATCTGTTCGCAGACTTCCTAAAGAGAAGTATCTCAAGGAGTGCGTTCAACAACAACTGCAATGA
Protein Sequence: MGRFSVNNNILWQIIALYKDKNSQREIVRKLEISKTCVQHTIKRFVETGSVKHKTRTGRPKRVTDRVKSKLIQIVRKNRSMTSREIANELEYSTQVKISAAHIRKILCKVNYKSRTAAKKPFITPKNAINRKRWCRERKNWTVQQWKKIVFSDESTFQLFPNSRRSVRRLPKEKYLKECVQQQLQ

Sequence clustering based on sequence similarity using MMseqs2