Basic Information

Insect: Sesamia inferens
Gene Symbol: RHOBTB1_1
Assembly: GCA_037179545.1
Location: CM073943.1:22638347-22675643[+]

Transcription Factor Domain

TF Family: BTB
Domain: zf-C2H2|ZBTB
PFAM: PF00651
TF Group: Zinc-Coordinating Group
Description: The BTB (for BR-C, ttk and bab) [6] or POZ (for Pox virus and Zinc finger) [1] domain is present near the N-terminus of a fraction of zinc finger (Pfam:PF00096) proteins and in proteins that contain the Pfam:PF01344 motif such as Kelch and a family of pox virus proteins. The BTB/POZ domain mediates homomeric dimerisation and in some instances heteromeric dimerisation [1]. The structure of the dimerised PLZF BTB/POZ domain has been solved and consists of a tightly intertwined homodimer. The central scaffolding of the protein is made up of a cluster of alpha-helices flanked by short beta-sheets at both the top and bottom of the molecule [2]. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes including N-CoR and SMRT [5, 3, 4]. The POZ or BTB domain is also known as BR-C/Ttk or ZiN.
Hmmscan Out: # of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc

1 21 5.9e-07 0.00039 22.2 0.2 3 44 256 296 254 317 0.84

2 21 6.3e-06 0.0041 18.9 0.4 47 109 394 455 385 456 0.83

3 21 3.9e-06 0.0025 19.6 0.0 7 55 481 526 476 534 0.82

4 21 0.0015 0.99 11.2 0.0 25 55 579 607 573 615 0.82

5 21 0.0015 0.99 11.2 0.0 25 55 660 688 654 696 0.82

6 21 0.0015 0.99 11.2 0.0 25 55 741 769 735 777 0.82

7 21 0.0015 0.99 11.2 0.0 25 55 822 850 816 858 0.82

8 21 0.0015 0.99 11.2 0.0 25 55 903 931 897 939 0.82

9 21 0.0015 0.99 11.2 0.0 25 55 984 1012 978 1020 0.82

10 21 0.0015 0.99 11.2 0.0 25 55 1065 1093 1059 1101 0.82

11 21 0.0015 0.99 11.2 0.0 25 55 1146 1174 1140 1182 0.82

12 21 0.0015 0.99 11.2 0.0 25 55 1227 1255 1221 1263 0.82

13 21 0.0015 0.99 11.2 0.0 25 55 1308 1336 1302 1344 0.82

14 21 0.0015 0.99 11.2 0.0 25 55 1389 1417 1383 1425 0.82

15 21 0.0015 0.99 11.2 0.0 25 55 1470 1498 1464 1506 0.82

16 21 0.0015 0.99 11.2 0.0 25 55 1551 1579 1545 1587 0.82

17 21 0.0015 0.99 11.2 0.0 25 55 1632 1660 1626 1668 0.82

18 21 0.0015 0.99 11.2 0.0 25 55 1713 1741 1707 1749 0.82

19 21 0.0015 0.99 11.2 0.0 25 55 1794 1822 1788 1830 0.82

20 21 0.0036 2.3 10.0 0.1 25 55 1875 1903 1869 1911 0.82

21 21 1.2e-14 7.5e-12 47.0 0.1 25 107 1956 2038 1950 2041 0.94

#	of	c-Evalue	i-Evalue	score	bias	hmm coord from	hmm coord to	ali coord from	ali coord to	env coord from	env coord to	acc
1	21	5.9e-07	0.00039	22.2	0.2	3	44	256	296	254	317	0.84
2	21	6.3e-06	0.0041	18.9	0.4	47	109	394	455	385	456	0.83
3	21	3.9e-06	0.0025	19.6	0.0	7	55	481	526	476	534	0.82
4	21	0.0015	0.99	11.2	0.0	25	55	579	607	573	615	0.82
5	21	0.0015	0.99	11.2	0.0	25	55	660	688	654	696	0.82
6	21	0.0015	0.99	11.2	0.0	25	55	741	769	735	777	0.82
7	21	0.0015	0.99	11.2	0.0	25	55	822	850	816	858	0.82
8	21	0.0015	0.99	11.2	0.0	25	55	903	931	897	939	0.82
9	21	0.0015	0.99	11.2	0.0	25	55	984	1012	978	1020	0.82
10	21	0.0015	0.99	11.2	0.0	25	55	1065	1093	1059	1101	0.82
11	21	0.0015	0.99	11.2	0.0	25	55	1146	1174	1140	1182	0.82
12	21	0.0015	0.99	11.2	0.0	25	55	1227	1255	1221	1263	0.82
13	21	0.0015	0.99	11.2	0.0	25	55	1308	1336	1302	1344	0.82
14	21	0.0015	0.99	11.2	0.0	25	55	1389	1417	1383	1425	0.82
15	21	0.0015	0.99	11.2	0.0	25	55	1470	1498	1464	1506	0.82
16	21	0.0015	0.99	11.2	0.0	25	55	1551	1579	1545	1587	0.82
17	21	0.0015	0.99	11.2	0.0	25	55	1632	1660	1626	1668	0.82
18	21	0.0015	0.99	11.2	0.0	25	55	1713	1741	1707	1749	0.82
19	21	0.0015	0.99	11.2	0.0	25	55	1794	1822	1788	1830	0.82
20	21	0.0036	2.3	10.0	0.1	25	55	1875	1903	1869	1911	0.82
21	21	1.2e-14	7.5e-12	47.0	0.1	25	107	1956	2038	1950	2041	0.94

Sequence Information

Coding Sequence: ATGGATAACGAGCAGCCCCACCAAGAGCTGGTAAAATGCGTGGTGGTAGGCGACACAGCAGTTGGCAAGACACGTCTCATATGTGCACGTGCCTGCAACAAGCATGTGTCGCTGTCACAGCTGATGACCACCCACGTGCCTACCGTGTGGGCTATTGACCAGTACCGGATATATAAAGATGTTCTAGAAAGATCTTGGGAAGTAGTAGACGGTGTGAATGTATCACTGCGCCTTTGGGATACTTTCGGTGATCATGAAAAGGACAGGAGATTTGCTTATGGAAGGTCTGATGTGGTTCTCCTATGTTTCTCAATAACAAACCCTGTGTCTTTGAGAAATTGTGGGGCTATGTGGTATCCTGAGATAAGACGATTCTGTCCAAACACACCAATTTTATTAGTGGGATGTAAAAACGATCTGCGTTATATGTACAGGGATGAAACCTACCTCAATTACTGTAAGGATCGCAGCCCTTTCATAAGGGCTCCGAGAAAAAGCGACCTGGTAATGCCGGATCAAGGGCGGGCACTAGCCCACGAGTTTGGAATATATTACTACGAGACCTCAGTGTTCACTTACTATGGGGTCAATGAGGTCTTTGAGAACGCCATCAGAGCTGCCTTGATAGCCAGGAGACAGCAGCGGTTCTGGATGACTAACTTGAAGCGGGTGCAGAGGCCACTTTTACAGGCACCATTCCGACCTCCTCGTCCGATGGAGCCTGAGGTGGCTGTCGTGAACAGCACTTACCTTGAGAACATGACCACTCTCATGCGACAACAGTATTTCTCGGATATGGTGATAATATGCGGTGCAAAGGGATTCCCAGTTCATCGGTTTATGATGGCGGCAGCATGCGAAGCCTTCCACCGTCTGCTGACAAACGAGTGCGTCAGCCTTTCTGCTGAGCTCGCTAGGAGTTCCAGTGAGAGCAGCATGGTGAGCAGTATGGGTGAGGCGACTGCCGGCGAGTTTAACGAGGACACCGAGTATTTGATACGACAGGACCAGGCTAAACAAATGAGGGTATGGGATCAGATCAAGCGTCGGTCTTCATGTCAGATCCTGCCTCTGAGCGACAGTTGTAAAAAACCTCCGGATTTATATAGAGAGGTCAACCATCCCGCTATCATCTCTATAAGAGTTGTTAagTGCGACAAGATCCAACATGCAACATCCCAAACACAGACTATAGTTACGATGAGCAAGTTAATCTCACAAACTGTCATGCAAGAGATCGTCAACTTTATATACACCGGCGCTTTAGATAGCAGTGCCTTCAAACAGCAGGAGATCAGACAGGCGGCCGAGCTGCTTGGCTTCCATGAACTCACCAAGCTCAGTCAGTTCATCCTGGACCAGCACCTGTTGTTCGACAAGGGATTCATGCTGCAGTTTCATACgCCGCTGGGAGCCCGCCTCCGCGACATGTACGTGGAGCGCGCGCTGTTCGCGGACGTGACGTTCGACCTCGACGACGGCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGAGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGAGACACTTCCGCGAGAGCACCTCCCGCGTGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGGTCAGTATAACTTGCTCAGCGTATCTATCAGACAACTAGTTGTCATCCACCTCGCGCACCGCGCCGTGCTCATGGCGCGCTGCGACCCCATGAAGGCCATGTTCCAGGGACACTTCCGCGAGAGCACCTCCCGCGTGATATCATTCCCCGGCGTCAAAATGTACGCCTTCCACATCCTGCTCAGCTACATCTATAGCGACAAAATACCCGCTGTGGACCCTAACAGATGCTTGGAACTCCTGGAGCTAGCCAACAGACTGTGCATGAACCGATTGGTGAACCTGGTGGAGGCTAGGGTCATAGATCAACTGCAGCAGAGAGACAGAGTGTGCGGTGATGATGATCAAGTCGTCGAGATAGCTCTGTCGCTGCTGGAGCCTGTCaagTTGCACAACGCCCACAACCTGGCGGCGTGGTGCACGTGGCGCCTGTGTGGGGCCTACGACCGAGTGTGTCGCGCCCGGAGCCTCAGTCAGGCCGAGCGGGACTACCTCTCCGACAACCGGTGGCCACCCGTCTGgtACGTGAAAGAGTTCGACTACTACCAGAAGTGCATGAACGAGCAGAGTAAAGAGCAGAAGGAGTTGAGACTCACCACGTCGCTGCAGAACAACCAGCAGACTGGCTGTCTTTGTTTTACCAGCAAGGTCCGGCGCGACAGCTCGCCCGCGCCTGACGTGACCACGGCGCTgtgtgccgccgccgccccgcacGACCCGCACGACCCGCACGACCCGCATCAGCCGCGTCTCTGA
Protein Sequence: MDNEQPHQELVKCVVVGDTAVGKTRLICARACNKHVSLSQLMTTHVPTVWAIDQYRIYKDVLERSWEVVDGVNVSLRLWDTFGDHEKDRRFAYGRSDVVLLCFSITNPVSLRNCGAMWYPEIRRFCPNTPILLVGCKNDLRYMYRDETYLNYCKDRSPFIRAPRKSDLVMPDQGRALAHEFGIYYYETSVFTYYGVNEVFENAIRAALIARRQQRFWMTNLKRVQRPLLQAPFRPPRPMEPEVAVVNSTYLENMTTLMRQQYFSDMVIICGAKGFPVHRFMMAAACEAFHRLLTNECVSLSAELARSSSESSMVSSMGEATAGEFNEDTEYLIRQDQAKQMRVWDQIKRRSSCQILPLSDSCKKPPDLYREVNHPAIISIRVVKCDKIQHATSQTQTIVTMSKLISQTVMQEIVNFIYTGALDSSAFKQQEIRQAAELLGFHELTKLSQFILDQHLLFDKGFMLQFHTPLGARLRDMYVERALFADVTFDLDDGIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPETLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQRHFRESTSRVTTSCHPPRAPRRAHGALRPHEGHVPGTLPREHLPRGQYNLLSVSIRQLVVIHLAHRAVLMARCDPMKAMFQGHFRESTSRVISFPGVKMYAFHILLSYIYSDKIPAVDPNRCLELLELANRLCMNRLVNLVEARVIDQLQQRDRVCGDDDQVVEIALSLLEPVKLHNAHNLAAWCTWRLCGAYDRVCRARSLSQAERDYLSDNRWPPVWYVKEFDYYQKCMNEQSKEQKELRLTTSLQNNQQTGCLCFTSKVRRDSSPAPDVTTALCAAAAPHDPHDPHDPHQPRL

Similar Transcription Factors

Sequence clustering based on sequence similarity using MMseqs2

100% Identity: -
90% Identity: -
80% Identity: -