Basic Information

Insect: Tipula unca
Gene Symbol: gcm
Assembly: GCA_951394425.1
Location: OX596381.1:186113459-186121676[+]

Transcription Factor Domain

TF Family: GCM
Domain: GCM domain
PFAM: PF03615
TF Group: Beta-Scaffold Factors
Description: GCM transcription factors are a family of proteins which contain a GCM motif. The GCM motif is a domain that has been identified in proteins belonging to a family of transcriptional regulators involved in fundamental developmental processes which comprise Drosophila melanogaster GCM and its mammalian homologues [PMID: 8962155, PMID: 9114061, PMID: 9580683, PMID: 10671510]. IN GCM transcription factors the N-terminal moiety contains a DNA-binding domain of 150 residues. Sequence conservation is highest in this GCM domain. In contrast, the C-terminal moiety contains one or two transactivating regions and is only poorly conserved.The GCM motif has been shown to be a DNA binding domain that recognises preferentially the nonpalindromic octamer 5'-ATGCGGGT-3' [PMID: 8962155, PMID: 9114061, PMID: 9580683]. The GCM motif contains many conserved basic amino acid residues, seven cysteine residues, and four histidine residues [PMID: 8962155]. The conserved cysteines are involved in shaping the overall conformation of the domain, in the process of DNA binding and in the redox regulation of DNA binding [PMID: 9580683]. The GCM domain as a new class of Zn-containing DNA-binding domain with no similarity to any other DNA-binding domain [PMID: 12682016]. The GCM domain consists of a large and a small domain tethered together by one of the two Zn ions present in the structure. The large and the small domains comprise five- and three-stranded beta-sheets, respectively, with three small helical segments packed against the same side of the two beta-sheets. The GCM domain exercises a novel mode of sequence-specific DNA recognition, where the five-stranded beta-pleated sheet inserts into the major groove of the DNA. Residues protruding from the edge strand of the beta-pleated sheet and the following loop and strand contact the bases and backbone of both DNA strands, providing specificity for its DNA target site.
Hmmscan Out: # of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc

1 3 1.4e-78 3.6e-74 248.7 6.0 1 140 59 198 59 198 0.99

2 3 0.44 1.1e+04 -2.3 0.2 100 114 263 277 224 295 0.57

3 3 2 5e+04 -4.6 6.7 13 41 458 486 450 500 0.80

#	of	c-Evalue	i-Evalue	score	bias	hmm coord from	hmm coord to	ali coord from	ali coord to	env coord from	env coord to	acc
1	3	1.4e-78	3.6e-74	248.7	6.0	1	140	59	198	59	198	0.99
2	3	0.44	1.1e+04	-2.3	0.2	100	114	263	277	224	295	0.57
3	3	2	5e+04	-4.6	6.7	13	41	458	486	450	500	0.80

Sequence Information

Coding Sequence: ATGCATCGGACGGGGGATATTTATGACCTCGAGACAATTATGGTCCTGTATTACGCGAGATCTATTTCACCAACGGCAATCAATAATCAACTTAATGGGGCTTTAATAAAAAGTCGATTAGCTACAGAATGGGATATAAACGATACAAATGTACCAAAAGTGTTGGATGGTGAATACGATGATTATTGCGAATGGGCGAATGGACATTGCCGATTTGTTTACTCCGTGTACAATGAGGATGCAAAGAAACATTCATCAGGATGGGCTATGCGAAATACTAACAATCATAATGTTAATATACTCAAAAAAAGCTGTTTGGGGGTGATATTATGTTCTGAAACGTGCCATCTACCGAATGGAGATCGTATTCATTTGCGGCCTGCAATATGTGATAAGGCCCGTAAAAAGCAACAGGGCAAGTCATGCCCGAATCGCAATTGTGgtggaattttagaaattcagccATGCCGAGGACATTGCGGATATCCTGTTACACATTTTTGGCGACACACCGATCATGGAATATTTTTCCAAGCGAAAGGTGTACACGACCATCCCAGGCCAGAGGCTAAAGGATCAAGCGAATCTAGACGTATATTAGGCGCGAGTGGTCGGAAAACTCGATCGCTCAATATGATTTTATCACGCAACTCAGCCATAGAGTCTAAGATATGTAAATTACGATTAAAACAAACCAAACATCAAACGGCAATAAATTCATATGAAAATCCAATCTCATATATCAACTCTAATATGACACATAACAGTTCAGTAAAtatgttgaataaaaaatcacaacaaCCATCCACCGATTGGTATTATAATAATACCGAAGCAATGACATACAATAATCATTACGCAAATCACAACCATATGATGGGGTATAGTGAAAATATACCCCAAGATTACGCACAGTATGGCGATCAAAATTATTGTCTGCAAAATCAGCAACCagcaaattataacaataataataatttttatacgaacACAGAGTACTTCTATCCGGAAGACATATTTCAATTGGATCAACCACTTAATACTAAATCAAGCGGTGACACAAATGGGCTAATAAATGCAACCACTACGCCGACACTATTGGATATGGAAAGTGGGACAATTCAACAGaagcaacaaaataataatacaggCGGTTTTACCgcataccaacaaaattggtatgatgTTGACATGAAATACGAAAGCTGTGACGATACAGTAAGTCTGACGAGTGGATCAAGTATAGTGGACGATCATTTTTACCAAAATAATGCCAAATTGAGCGCAAACCATGTTATAACCGattttaattcgattaaaatttttaatcaaaatattatcgaaaatCCCAGTAGTAATAACAATGATTACTATTACAATTATAAtcatagtaataacaataataatgcgTATAATTACGAGTACGCTGctcagaataataataacaataataataatgcgaACAGTTGGGACGCCACCGGTGAAAATTGTTACggccaattggaaaattttgtgaaCTCAAATCAAGTTTATTCGATGGCTCATTAA
Protein Sequence: MHRTGDIYDLETIMVLYYARSISPTAINNQLNGALIKSRLATEWDINDTNVPKVLDGEYDDYCEWANGHCRFVYSVYNEDAKKHSSGWAMRNTNNHNVNILKKSCLGVILCSETCHLPNGDRIHLRPAICDKARKKQQGKSCPNRNCGGILEIQPCRGHCGYPVTHFWRHTDHGIFFQAKGVHDHPRPEAKGSSESRRILGASGRKTRSLNMILSRNSAIESKICKLRLKQTKHQTAINSYENPISYINSNMTHNSSVNMLNKKSQQPSTDWYYNNTEAMTYNNHYANHNHMMGYSENIPQDYAQYGDQNYCLQNQQPANYNNNNNFYTNTEYFYPEDIFQLDQPLNTKSSGDTNGLINATTTPTLLDMESGTIQQKQQNNNTGGFTAYQQNWYDVDMKYESCDDTVSLTSGSSIVDDHFYQNNAKLSANHVITDFNSIKIFNQNIIENPSSNNNDYYYNYNHSNNNNNAYNYEYAAQNNNNNNNNANSWDATGENCYGQLENFVNSNQVYSMAH

Similar Transcription Factors

Sequence clustering based on sequence similarity using MMseqs2

100% Identity: iTF_01455435;
90% Identity: -
80% Identity: -