Basic Information

Gene Symbol
Mad
Assembly
GCA_963942525.1
Location
OZ012636.1:13586657-13588081[+]

Transcription Factor Domain

TF Family
MH1
Domain
MH1 domain
PFAM
PF03165
TF Group
Unclassified Structure
Description
The MH1 (MAD homology 1) domain is found at the amino terminus of MAD related proteins such as Smads. This domain is separated from the MH2 domain by a non-conserved linker region. The crystal structure of the MH1 domain shows that a highly conserved 11 residue beta hairpin is used to bind the DNA consensus sequence GNCN in the major groove, shown to be vital for the transcriptional activation of target genes. Not all examples of MH1 can bind to DNA however. Smad2 cannot bind DNA and has a large insertion within the hairpin that presumably abolishes DNA binding. A basic helix (H2) in MH1 with the nuclear localisation signal KKLKK has been shown to be essential for Smad3 nuclear import. Smads also use the MH1 domain to interact with transcription factors such as Jun, TFE3, Sp1, and Runx [2, 1].
Hmmscan Out
# of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc
1 1 1e-45 4.2e-42 142.4 1.3 2 103 45 145 44 145 0.97

Sequence Information

Coding Sequence
atGGAAACGGACGACGTTGAATCATCTAGTAGCAGCACTATGTCTagtttaaatagtttattttcatttaccaGTCCAGcggtgaaaaaattattaggtTGGAAACAGGGTGATGAAGAGGAAAAATGGGCAGAAAAAGCGGTTGATAGTTtagttaaaaaactaaaaaaacgtAAAGGTGCTATTGAGGAATTGGAACGTGCATTATCGTGTCCAGGACAACCTTCAAAGTGTGTCACAATACCAAGATCATTAGATGGAAGATTACAAgtATCACATCGAAAAGGATTGCCCCATGTTATATATTGTCGTGTATGGCGTTGGCCAGATTTACAGAGTCATCATGAATTAAAACCACTCGAAATATGTCAATATCCGTTTAGTGCTAAGCAAAAAGAAGTTTGTATTAATCCGTATCATTATAAGCGTGTAGAGAGCCCAGTATTGCCTCCAGTTTTGGTTCCGCGTCATTCAGAGTTTGCACCAGGACATTCTCTGCTACCATTTCAGCAAATGCCAGAACCAAATATGCCGCATAATGTTAGTTATTCAACAGCAGGTTTTAATTCACATCTAAGTCCGAACAGTCCAATGTCATCTGTGGCTAGTCCTGGTTCTGTTAATTCAAATCCCACAAGTCCATATGGATCTTTGCCGGAAACACCACCACCAGCTTATAGTCCTCCAGAAGATGCAAATTCAAATAATCAACCAGACACTGGAATGGATTCTCAAATGGGTGAAGTAGCACCAGTTAGCTACCAAGAACCGCCATACTGGGCGAGTATAGCCTATTACGAGCTGAATTGTCGTGTCGGTGAAGTATTTCATTGTACAACACACTCTGTCATTGTTGACGGCTTTACAAATCCATCAAACAATTCGGATAGATTCTGTCTTGGACAATTGAGTAATGTTAATCGAAATAGCACAATTGAAAATACAAGACGGCACATTGGTAAAGGTGTTCACTTGTATTATGTCGGCGGTGAAGTGTACGCCGAATGTTTATCCGATTCAGCAATATTTGTACAAAGTAGAAATTGTAATCATCATCACGGTTTTCATCCAAGTACTGTGTGTAAAATACCGCCAGGATGTTCattgaaaattttcaataatcaaGAATTTGCACAATTATTATCACAATCGGTGAATCATGGTTTTGAGGCTGTAtatgaattaacaaaaatgtgCACAATCCGTATGAGTTTTGTTAAAGGTTGGGGTGCAGAATATCATCGTCAAGATGTAACATCCACACCATGTTGGATCGAAATACATTTACATGGACCATTGCAGTGGTTAGATAAAGTTTTAACACAAATGGGTTCACCACACAATGCAATTAGTTCAGTatcttaa
Protein Sequence
METDDVESSSSSTMSSLNSLFSFTSPAVKKLLGWKQGDEEEKWAEKAVDSLVKKLKKRKGAIEELERALSCPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLEICQYPFSAKQKEVCINPYHYKRVESPVLPPVLVPRHSEFAPGHSLLPFQQMPEPNMPHNVSYSTAGFNSHLSPNSPMSSVASPGSVNSNPTSPYGSLPETPPPAYSPPEDANSNNQPDTGMDSQMGEVAPVSYQEPPYWASIAYYELNCRVGEVFHCTTHSVIVDGFTNPSNNSDRFCLGQLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSAIFVQSRNCNHHHGFHPSTVCKIPPGCSLKIFNNQEFAQLLSQSVNHGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNAISSVS

Similar Transcription Factors

Sequence clustering based on sequence similarity using MMseqs2

100% Identity
iTF_01276266; iTF_00733121; iTF_00731502; iTF_00743745; iTF_00742804; iTF_00201666; iTF_00601794; iTF_00533802; iTF_00585199; iTF_00059459; iTF_00398085; iTF_00397294; iTF_00721262; iTF_00841078; iTF_01497421; iTF_00372931; iTF_01207363; iTF_00098404; iTF_00414308; iTF_01191204; iTF_01191906; iTF_01363940; iTF_01449636; iTF_01448579; iTF_01447588; iTF_01450604; iTF_01445681; iTF_01446616; iTF_01488580; iTF_00189957; iTF_00082945; iTF_00303809; iTF_01357954; iTF_01563779; iTF_00191364; iTF_00081229; iTF_00091247; iTF_00192818; iTF_01562898; iTF_00190675; iTF_01189429; iTF_00992599; iTF_00192092; iTF_01045469; iTF_01055229; iTF_00193566; iTF_01505406; iTF_00285445; iTF_00727397; iTF_01564677; iTF_01288986; iTF_00890981; iTF_00891885; iTF_00890131; iTF_00061947; iTF_00957061; iTF_01195269; iTF_00046150; iTF_00046979; iTF_00092166; iTF_00050951; iTF_00265697; iTF_00262662; iTF_00263469; iTF_01299182; iTF_00266457; iTF_01508865; iTF_01103555; iTF_01102055; iTF_01102799; iTF_01100593; iTF_01101332; iTF_01129350; iTF_00828781; iTF_00439475; iTF_00829503; iTF_01057648; iTF_01056818; iTF_01055991; iTF_00615559; iTF_00481538; iTF_00566087; iTF_00550249; iTF_00579978; iTF_00545963; iTF_00366509; iTF_00538961; iTF_00488018; iTF_00503987; iTF_00475926; iTF_00477334; iTF_00614903; iTF_00014021; iTF_00592389; iTF_00482977; iTF_00480163; iTF_00514883; iTF_00531651; iTF_00486564; iTF_00606308; iTF_00915334; iTF_00503271; iTF_00517704; iTF_00527304; iTF_00539656; iTF_00555849; iTF_00578537; iTF_00604841; iTF_00914505; iTF_00483690; iTF_00515594; iTF_00543114; iTF_00533099; iTF_00612075; iTF_00612811; iTF_00919819; iTF_00603341; iTF_00617645; iTF_00619149; iTF_00916237; iTF_00504686; iTF_00555086; iTF_00588142; iTF_00607790; iTF_00473776; iTF_00494384; iTF_00541072; iTF_00591696; iTF_00596656; iTF_00523697; iTF_00611346; iTF_00919058; iTF_00484435; iTF_00525876; iTF_00918132; iTF_00488661; iTF_00491510; iTF_00501046; iTF_00571158; iTF_00571894; iTF_00580728; iTF_00590968; iTF_00480857; iTF_00590233; iTF_00601049; iTF_00471610; iTF_00574810; iTF_00581530; iTF_00489371; iTF_00505403; iTF_00917211; iTF_00487292; iTF_00507612; iTF_00536765; iTF_00600317; iTF_00565374; iTF_00604094; iTF_00478015; iTF_00548063; iTF_00602591; iTF_00474496; iTF_00563904; iTF_00644101; iTF_00812773; iTF_01224263; iTF_00160699; iTF_00363261; iTF_00643219; iTF_01297399; iTF_01003527; iTF_01006068; iTF_01004251; iTF_01005088; iTF_01521095; iTF_00319688; iTF_00199092; iTF_00320603; iTF_01257126; iTF_01250088; iTF_00346735; iTF_01352657; iTF_00593832; iTF_00485133; iTF_00561754; iTF_00442729; iTF_00442728; iTF_00687177; iTF_00716713; iTF_01095817; iTF_01163945; iTF_01434953; iTF_00348203; iTF_01059883; iTF_01465195; iTF_01466220; iTF_01467984; iTF_01469691; iTF_00857644; iTF_01553228; iTF_00336737; iTF_00485857; iTF_00551585; iTF_00601793; iTF_00613505; iTF_01324897; iTF_00616194; iTF_00359459; iTF_00478723; iTF_00516259; iTF_00548803; iTF_00558659; iTF_00573315; iTF_00577059; iTF_01550275; iTF_00512004; iTF_00528804; iTF_00616900; iTF_00832164; iTF_00473061; iTF_00498149; iTF_00525125; iTF_00542437; iTF_00562469; iTF_00563173; iTF_00566789; iTF_00584421; iTF_01552453; iTF_00495110; iTF_00518438; iTF_00521350; iTF_00530950; iTF_00537510; iTF_00595942; iTF_00598150; iTF_00497383; iTF_01321191; iTF_00564648; iTF_00589544; iTF_00804392; iTF_01554696; iTF_00509097; iTF_00533801; iTF_00544489; iTF_00614185; iTF_00199882; iTF_00501802; iTF_00508330; iTF_00511274; iTF_00553034; iTF_00556522; iTF_00593098; iTF_01553962; iTF_00199883; iTF_00582955; iTF_00588843; iTF_00595243; iTF_00900587; iTF_00526572; iTF_00560268; iTF_00339149; iTF_00510541; iTF_00522957; iTF_00528045; iTF_00549549; iTF_00554381; iTF_00618413; iTF_01324109; iTF_01559757; iTF_00475214; iTF_00476622; iTF_00512718; iTF_00535310; iTF_00540366; iTF_00567534; iTF_00579278; iTF_00582254; iTF_00585198; iTF_00585966; iTF_00597358; iTF_00803598; iTF_00904750; iTF_01319758; iTF_00493647; iTF_00609167; iTF_00519920; iTF_00532368; iTF_00534576; iTF_00568976; iTF_00586672; iTF_00805968; iTF_01327888; iTF_01555408; iTF_00506865; iTF_00337522; iTF_00538226; iTF_00552306; iTF_01320466; iTF_01322642; iTF_00495859; iTF_00500307; iTF_00496596; iTF_00509812; iTF_00545236; iTF_00569684; iTF_00572596; iTF_00520629; iTF_00470870; iTF_00482230; iTF_00513462; iTF_00530262; iTF_00577782; iTF_01557558; iTF_00607063; iTF_01559031; iTF_00479480; iTF_00490776; iTF_00498872; iTF_00557208; iTF_00570392; iTF_00576344; iTF_00610648; iTF_01319032; iTF_01570232; iTF_00568249; iTF_00608441; iTF_00609881; iTF_01383609; iTF_00335898; iTF_00502522; iTF_00529562; iTF_00802842; iTF_01326394; iTF_00506107; iTF_00516987; iTF_00524411; iTF_00557915; iTF_00522172; iTF_00546652; iTF_01325660; iTF_00559485; iTF_00619837; iTF_00492958; iTF_00514174; iTF_00574046; iTF_00805182; iTF_01321918; iTF_01327166; iTF_00499583; iTF_00541759; iTF_00575583; iTF_00601791; iTF_00338336; iTF_00543744; iTF_00583680; iTF_00598880; iTF_00599584; iTF_00601792; iTF_00605568; iTF_01051201; iTF_00331522; iTF_00258881; iTF_00350177; iTF_00435553; iTF_01231585; iTF_01176933; iTF_00045350; iTF_01019430; iTF_01236678; iTF_00653543; iTF_01019431; iTF_00716712; iTF_00199881; iTF_00679498; iTF_00816554; iTF_00900586; iTF_01201610; iTF_01259211; iTF_01162252; iTF_01374956; iTF_01074477; iTF_01194415; iTF_01261007; iTF_01174256; iTF_01315504; iTF_01313009; iTF_01313810; iTF_00817428; iTF_00892756; iTF_00741877; iTF_00892757; iTF_01398487; iTF_01427479; iTF_00655321; iTF_00921433; iTF_00922105; iTF_01235737; iTF_01314681; iTF_00259770; iTF_00371034; iTF_01237658; iTF_01397434; iTF_00975611; iTF_01165531; iTF_01397435; iTF_00899580; iTF_01109340; iTF_01138012; iTF_01175100; iTF_01138013; iTF_00760094; iTF_01399455; iTF_01374080; iTF_01376640; iTF_00384578; iTF_01115015; iTF_01115016; iTF_01114262; iTF_00384579; iTF_00670947; iTF_00671592; iTF_00672848; iTF_01363939; iTF_00492228; iTF_00490078; iTF_00991812; iTF_00688673; iTF_01356132; iTF_01521892; iTF_01521094; iTF_01044716; iTF_01522670; iTF_00310256; iTF_00967189; iTF_00976485; iTF_00335046; iTF_00426344; iTF_00664840; iTF_01396522; iTF_01318252; iTF_00664078; iTF_00974671; iTF_00694641; iTF_00984241; iTF_00188117; iTF_00427157; iTF_00693845; iTF_01356885; iTF_01430853; iTF_01489381; iTF_01541445; iTF_01542216; iTF_00062790; iTF_00877094; iTF_00999590; iTF_00322469; iTF_01043785; iTF_01088362; iTF_00793525; iTF_01127420; iTF_01375840; iTF_01457350; iTF_00750471; iTF_00748229; iTF_00749704; iTF_00748971; iTF_00747477; iTF_00746716; iTF_00997735; iTF_00999591; iTF_00322470; iTF_01251079; iTF_01486183; iTF_00061128; iTF_00118696; iTF_00283560; iTF_00313480; iTF_00312615; iTF_00314312; iTF_00315976; iTF_00311058; iTF_01223432; iTF_01253896; iTF_01253897; iTF_01253898; iTF_01253900; iTF_00724797; iTF_00118697; iTF_00972201; iTF_00257829; iTF_01471433; iTF_00334090; iTF_00665642; iTF_01471434; iTF_00339883; iTF_00650891; iTF_01023019; iTF_01132203; iTF_01404907; iTF_01164908; iTF_00233901; iTF_00234795; iTF_00311821; iTF_01116461; iTF_01415021; iTF_01181161; iTF_00024622; iTF_00362542; iTF_00797742; iTF_01211950; iTF_01323374; iTF_00324399; iTF_00624752; iTF_01551728; iTF_01550995; iTF_01558293; iTF_01556830; iTF_01431921; iTF_00315187; iTF_01470515; iTF_01518995; iTF_00402772; iTF_00424660; iTF_00793524; iTF_01461909; iTF_01461910; iTF_00948759; iTF_00240758; iTF_00948758; iTF_01461908; iTF_00241463; iTF_00351864; iTF_00979352; iTF_01200906; iTF_00979351; iTF_01378392; iTF_00210809; iTF_00211631; iTF_00672217; iTF_01348025; iTF_01556121; iTF_00587396; iTF_00519162; iTF_00389626; iTF_00753754; iTF_00901498; iTF_01132923; iTF_00352508; iTF_00899581; iTF_00893886; iTF_00203551; iTF_00202586; iTF_01300895; iTF_01300037; iTF_00440352; iTF_00454816; iTF_00453501; iTF_01549568; iTF_00097069; iTF_00094480; iTF_00857645; iTF_01059884; iTF_01479437; iTF_01301637; iTF_01478827; iTF_01500365; iTF_01537965; iTF_00993541; iTF_00401710; iTF_00400572; iTF_00734575; iTF_00939553; iTF_01414178; iTF_01412326; iTF_00159111; iTF_00938695; iTF_00060212; iTF_01411511; iTF_01200153; iTF_01413181; iTF_01303359; iTF_00452578; iTF_00877858; iTF_01087202; iTF_01207362; iTF_00343184; iTF_00652720; iTF_00127459; iTF_00125928; iTF_00128202; iTF_00128978; iTF_00129746; iTF_00126716; iTF_00287235; iTF_01524035; iTF_00174180; iTF_01048228; iTF_00175751; iTF_01048909; iTF_00175031; iTF_00719418; iTF_00451940; iTF_00733956; iTF_01420935; iTF_00873802; iTF_01417709; iTF_01418353; iTF_00982293; iTF_01420279; iTF_01419000; iTF_00982970; iTF_01419644; iTF_00306270; iTF_01269060; iTF_01267566; iTF_01271216; iTF_01268274; iTF_00073733; iTF_00378021; iTF_00379620; iTF_00378759; iTF_00381064; iTF_00381840; iTF_00380286; iTF_00438757; iTF_01513477; iTF_01000550; iTF_01266886; iTF_01270502; iTF_01380007; iTF_01190344; iTF_00143879; iTF_01233000; iTF_01234347; iTF_01498223; iTF_01232403; iTF_01235029; iTF_01233658; iTF_00309513; iTF_00645961; iTF_00938025; iTF_01273097; iTF_00168426; iTF_00351018; iTF_01216778; iTF_01269802; iTF_01364650; iTF_00905425; iTF_00058708; iTF_00057949; iTF_00086283; iTF_00718571; iTF_00718570; iTF_01514827; iTF_01514159; iTF_01512188; iTF_01512832; iTF_00730060; iTF_01405698; iTF_01520229; iTF_00264998; iTF_00109831; iTF_00868103; iTF_01015815; iTF_00385320; iTF_01245224; iTF_00867401; iTF_00015371; iTF_00016683; iTF_00730059; iTF_00181964; iTF_01254797; iTF_01355309; iTF_01423661; iTF_01476173; iTF_00765584; iTF_00264274; iTF_00730738; iTF_01422931; iTF_01099189; iTF_01407285; iTF_00885110; iTF_01354614; iTF_00898743; iTF_01421559; iTF_01476919; iTF_00417560; iTF_00181305; iTF_00729305; iTF_01408219; iTF_01422235; iTF_01077468; iTF_01409116; iTF_00280605; iTF_01229022; iTF_01477566; iTF_00182623; iTF_00279967; iTF_01406495; iTF_00014720; iTF_00868800; iTF_01523360; iTF_01110124; iTF_01113432; iTF_01112601; iTF_01111772; iTF_00459758; iTF_00414307; iTF_01001262; iTF_01089998; iTF_01274479; iTF_01120208; iTF_00738353; iTF_00738352; iTF_00739089; iTF_01036209; iTF_00286339; iTF_00326387; iTF_00728659; iTF_00629108; iTF_00773812; iTF_01037012; iTF_01487032; iTF_01035424; iTF_01379220; iTF_01560436; iTF_01539605; iTF_00983607; iTF_01273783; iTF_00676024; iTF_00708536; iTF_00304482; iTF_00769624; iTF_01381336; iTF_01380611; iTF_00714385; iTF_00712859; iTF_00713664; iTF_00707865; iTF_01384729; iTF_01131565; iTF_00845274; iTF_00968826; iTF_00969619; iTF_00996346; iTF_00997040; iTF_00754651; iTF_01428283; iTF_00317468; iTF_00316787; iTF_00668444; iTF_01306460; iTF_00463997; iTF_01219100; iTF_00630525; iTF_01483869; iTF_01481566; iTF_01484638; iTF_01482400; iTF_01002078; iTF_01308030; iTF_01308697; iTF_00047967; iTF_00079935; iTF_00305055; iTF_00633631; iTF_00391295; iTF_01468864; iTF_01213327; iTF_00757328; iTF_01214800; iTF_01214058; iTF_00652721; iTF_01540337; iTF_01540339; iTF_00023794; iTF_01086308; iTF_00179361; iTF_00675146; iTF_01024957; iTF_00299071; iTF_00800199; iTF_00799281; iTF_00800981; iTF_01366985; iTF_00815812; iTF_01218340; iTF_00815109; iTF_00158058; iTF_00158059; iTF_00751211; iTF_01007374; iTF_01042702; iTF_01012308; iTF_00897814; iTF_01008540; iTF_00839456; iTF_01002802; iTF_01540338; iTF_01110963; iTF_00691941; iTF_00690184; iTF_00691094; iTF_00692918; iTF_01286419; iTF_00306920; iTF_00797210; iTF_01272052; iTF_00100309; iTF_00594549; iTF_01265781; iTF_00082134; iTF_00092822; iTF_00450998; iTF_01168433; iTF_01160471; iTF_00251353; iTF_00131290; iTF_00132614; iTF_00131960; iTF_01314682; iTF_01395842; iTF_01054340; iTF_01457349; iTF_01051942; iTF_01459699; iTF_01053173; iTF_01455770; iTF_00456027; iTF_01458395; iTF_01454848; iTF_01382085; iTF_01382921; iTF_00768303; iTF_00028758; iTF_01275286; iTF_00766551; iTF_01225688; iTF_00469657; iTF_01104381; iTF_01315505; iTF_01464182; iTF_01463277; iTF_00009050; iTF_00022816; iTF_00792924; iTF_00099003; iTF_00095651; iTF_00106404; iTF_00103016; iTF_00107689; iTF_00106988; iTF_00108396; iTF_00095076; iTF_00099661; iTF_00101679; iTF_00093458; iTF_00105142; iTF_00097776; iTF_00097777; iTF_00104375; iTF_01011223; iTF_00105832; iTF_00103674; iTF_00109147; iTF_00096388; iTF_00102364; iTF_00094013; iTF_00100959; iTF_00246331; iTF_00245410; iTF_00368123; iTF_00368804; iTF_00884059; iTF_00145593; iTF_00386361; iTF_01365468; iTF_01492892; iTF_01493590; iTF_00963850; iTF_00088001; iTF_00864641; iTF_01424385; iTF_00227970; iTF_00228674; iTF_00961826; iTF_00085379; iTF_00142467; iTF_00393603; iTF_00141828; iTF_00756644; iTF_00223877; iTF_01065573; iTF_00220582; iTF_00226598; iTF_00230694; iTF_00862567; iTF_01069671; iTF_00360905; iTF_00863273; iTF_01454089; iTF_00684170; iTF_00860394; iTF_00219115; iTF_00224555; iTF_00860395; iTF_00229356; iTF_01068300; iTF_00084557; iTF_00141229; iTF_00216397; iTF_00227277; iTF_00140590; iTF_00183950; iTF_00221263; iTF_00215024; iTF_00230032; iTF_00233232; iTF_00866705; iTF_00962513; iTF_01068977; iTF_01071025; iTF_00217075; iTF_01066259; iTF_01169172; iTF_00217750; iTF_00225238; iTF_00684790; iTF_00861878; iTF_00963191; iTF_00183288; iTF_00223192; iTF_00360216; iTF_00625496; iTF_00760984; iTF_00861144; iTF_01123064; iTF_00219801; iTF_00231321; iTF_00861145; iTF_00088826; iTF_00218426; iTF_00087163; iTF_00762555; iTF_00866011; iTF_00118012; iTF_00964477; iTF_01067613; iTF_00215710; iTF_00225918; iTF_01255550; iTF_00763231; iTF_00222528; iTF_01066944; iTF_01070368; iTF_01108413; iTF_00849822; iTF_00090424; iTF_00345088; iTF_00881248; iTF_01130817; iTF_00253571; iTF_01474691; iTF_00770375; iTF_01473929; iTF_00252074; iTF_00298314; iTF_00255084; iTF_01198668; iTF_00296784; iTF_01199408; iTF_00292273; iTF_00294481; iTF_00296019; iTF_00297555; iTF_00798579; iTF_00252820; iTF_00291540; iTF_00295252; iTF_00292951; iTF_01395127; iTF_00254328; iTF_00293724; iTF_01473182; iTF_01475454; iTF_00880452; iTF_01391108; iTF_01392773; iTF_01391978; iTF_01390219; iTF_01393523; iTF_01389431; iTF_00011099; iTF_01394304; iTF_00064456; iTF_00089549; iTF_00460527; iTF_00200753; iTF_00879636; iTF_00676726; iTF_00882966; iTF_00882030; iTF_00980123; iTF_01046272; iTF_00344234;
80% Identity
-