Basic Information

Insect: Phlebotomus argentipes
Gene Symbol: gcm_2
Assembly: GCA_947086385.1
Location: CAMTYR010000041.1:10004446-10006530[+]

Transcription Factor Domain

TF Family: GCM
Domain: GCM domain
PFAM: PF03615
TF Group: Beta-Scaffold Factors
Description: GCM transcription factors are a family of proteins which contain a GCM motif. The GCM motif is a domain that has been identified in proteins belonging to a family of transcriptional regulators involved in fundamental developmental processes which comprise Drosophila melanogaster GCM and its mammalian homologues [PMID: 8962155, PMID: 9114061, PMID: 9580683, PMID: 10671510]. IN GCM transcription factors the N-terminal moiety contains a DNA-binding domain of 150 residues. Sequence conservation is highest in this GCM domain. In contrast, the C-terminal moiety contains one or two transactivating regions and is only poorly conserved.The GCM motif has been shown to be a DNA binding domain that recognises preferentially the nonpalindromic octamer 5'-ATGCGGGT-3' [PMID: 8962155, PMID: 9114061, PMID: 9580683]. The GCM motif contains many conserved basic amino acid residues, seven cysteine residues, and four histidine residues [PMID: 8962155]. The conserved cysteines are involved in shaping the overall conformation of the domain, in the process of DNA binding and in the redox regulation of DNA binding [PMID: 9580683]. The GCM domain as a new class of Zn-containing DNA-binding domain with no similarity to any other DNA-binding domain [PMID: 12682016]. The GCM domain consists of a large and a small domain tethered together by one of the two Zn ions present in the structure. The large and the small domains comprise five- and three-stranded beta-sheets, respectively, with three small helical segments packed against the same side of the two beta-sheets. The GCM domain exercises a novel mode of sequence-specific DNA recognition, where the five-stranded beta-pleated sheet inserts into the major groove of the DNA. Residues protruding from the edge strand of the beta-pleated sheet and the following loop and strand contact the bases and backbone of both DNA strands, providing specificity for its DNA target site.
Hmmscan Out: # of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc

1 3 8.2e-77 5.1e-73 243.0 2.6 1 140 36 176 36 176 0.98

2 3 0.36 2.3e+03 -2.1 0.0 74 95 228 249 209 274 0.75

3 3 1.4 8.7e+03 -4.0 0.1 22 38 522 538 516 542 0.72

#	of	c-Evalue	i-Evalue	score	bias	hmm coord from	hmm coord to	ali coord from	ali coord to	env coord from	env coord to	acc
1	3	8.2e-77	5.1e-73	243.0	2.6	1	140	36	176	36	176	0.98
2	3	0.36	2.3e+03	-2.1	0.0	74	95	228	249	209	274	0.75
3	3	1.4	8.7e+03	-4.0	0.1	22	38	522	538	516	542	0.72

Sequence Information

Coding Sequence: ATGTCGTCTTTTTTCACCGGGAATCCAGTAATGATCAGCAAAGAGCGCATTATCAAGGATTGGGACATCAATGACACGAATGTTCCCATTATCAGTGACTCGGATTACGATGACTTTTGCGAGTGGGCGGATGGTCATGTGCGACTCGTCTATCCTGCCACGAATGAGGACGCCAAGAAGCACACATCCGGATGGGCGATGCGGAATACGAACAATCACAATGTGAACATCCTCAAGAAGAGCTGCCTCGGCGTCCTCATTTGCTCAGCGGGTTGTATTTTGCCCCATGGCGGGCGGGTGCACCTGCGCCCAGCCATTTGCGACAAGGCGCGCAAGAAGCAGCAGGGAAAGCCCTGTCCCAATCGCGCCTGCAAGGCAGGAAGACTGGAGATTCTGCCGTGCCGCGGGCACTGTGGCTACCCAGTGACCCACTTTTGGCGACACACCGAGAATGCCATCTTCTTCCAAGCCAAGGGCGTTCACGACCATCCGCGTCCCGAGCCAAAGAGCTCCAGCGAAACCCGCAGGAGCCTCGGTAGCGGCCGAAGAGTCCGCGGATTGGCAGTACTTCTGGCCAGAGACGCAGCTCTTGGGAATAAGCTGCTTTCCTTGCGACAGTCCAAGAAAGCGCCCAAATTACCCAGCACAGACATGACGGGACCTCCACCTCTTATCCCGGACTCAAATGCAGGAGACTCTTCATCATGCCTGTGTCCATCGCTCAACTGTACCTGTCGCCTGATGAGCAACATTCACCCTCAAGCTCCAACGCAGAACTTCACTCAGACGCCCACGCACTTTGGCTACCCGGAGTCATTCGGTGTGTCCATCATGAGTCCTCAGCAGCCGCCACAGCAGCCATCCAACTACTGGAGTCACTCGCAGACGCAATTCTCGGGCCTGCAGTTTCCCGAGAGCGGCAAACCTTATCTAGCATACAGCGACTGTTCAGTGGAAATGAGCGCGAACAATCAAATGGGGCTGGAAATGGCGAGTCAATCGGATTTGTATCAGTTTTCGCCCCTCTCGGGGGATCTTTTCCAGCCAGAGGAGATATTTCAGCTGGATCAGCCACTGAAGCCGCCGGGAGGTGTGACAAACTCCGCATCGCCGCCCACATTGCTCGACTTGGGCAGCGGAACAATTCAGCAGCAGAAGGGAATTTTCCCAGACGAAGAGAGCACTAATAATAGCTCATCGAGTCGAAACAACGAATCTAGTCCTCACCACTACGAGCCTGCTATCTTCACGCAGGGACATTTTCACAACAACAACAACCAGAGCCACTCTGGGTACTTCTGCGACGTCTCGAGTCACAATGAGCCGCAGATGTTTGGCAATCAACTCGAGGACGGGCGATTCGCGAAATCTGAGAACTTCTACTCAATGAGCGGATATGAGATGCCGAATGGCATGGACATTGGCAATGGATATCGCATGAGAAGGCGCAATGAGGACATCACAGAGAGATTGGATCTCTACGCACAGCCACAAGCTGTATCGTTGGACAGAGGATTGCAGGAATCAACAGCTTACTATGATATCACACCCGCGCAGTATTCGCGCGCTTCCTTCCACTCTCACGAGTACGCTGTGCCAAATGCCAACAATAATCACATCATGATGCCCCAGGACGCTTCTCAGGCCATCGAGAACAACATGCAGTTTACAATTTCCCTTGGCGATCACAATTAA
Protein Sequence: MSSFFTGNPVMISKERIIKDWDINDTNVPIISDSDYDDFCEWADGHVRLVYPATNEDAKKHTSGWAMRNTNNHNVNILKKSCLGVLICSAGCILPHGGRVHLRPAICDKARKKQQGKPCPNRACKAGRLEILPCRGHCGYPVTHFWRHTENAIFFQAKGVHDHPRPEPKSSSETRRSLGSGRRVRGLAVLLARDAALGNKLLSLRQSKKAPKLPSTDMTGPPPLIPDSNAGDSSSCLCPSLNCTCRLMSNIHPQAPTQNFTQTPTHFGYPESFGVSIMSPQQPPQQPSNYWSHSQTQFSGLQFPESGKPYLAYSDCSVEMSANNQMGLEMASQSDLYQFSPLSGDLFQPEEIFQLDQPLKPPGGVTNSASPPTLLDLGSGTIQQQKGIFPDEESTNNSSSSRNNESSPHHYEPAIFTQGHFHNNNNQSHSGYFCDVSSHNEPQMFGNQLEDGRFAKSENFYSMSGYEMPNGMDIGNGYRMRRRNEDITERLDLYAQPQAVSLDRGLQESTAYYDITPAQYSRASFHSHEYAVPNANNNHIMMPQDASQAIENNMQFTISLGDHN

Similar Transcription Factors

Sequence clustering based on sequence similarity using MMseqs2

100% Identity: -
90% Identity: -
80% Identity: -