Basic Information

Insect: Orius laevigatus
Gene Symbol: gcm
Assembly: GCA_018703685.1
Location: JAGWEN010000077.1:58031-66091[+]

Transcription Factor Domain

TF Family: GCM
Domain: GCM domain
PFAM: PF03615
TF Group: Beta-Scaffold Factors
Description: GCM transcription factors are a family of proteins which contain a GCM motif. The GCM motif is a domain that has been identified in proteins belonging to a family of transcriptional regulators involved in fundamental developmental processes which comprise Drosophila melanogaster GCM and its mammalian homologues [PMID: 8962155, PMID: 9114061, PMID: 9580683, PMID: 10671510]. IN GCM transcription factors the N-terminal moiety contains a DNA-binding domain of 150 residues. Sequence conservation is highest in this GCM domain. In contrast, the C-terminal moiety contains one or two transactivating regions and is only poorly conserved.The GCM motif has been shown to be a DNA binding domain that recognises preferentially the nonpalindromic octamer 5'-ATGCGGGT-3' [PMID: 8962155, PMID: 9114061, PMID: 9580683]. The GCM motif contains many conserved basic amino acid residues, seven cysteine residues, and four histidine residues [PMID: 8962155]. The conserved cysteines are involved in shaping the overall conformation of the domain, in the process of DNA binding and in the redox regulation of DNA binding [PMID: 9580683]. The GCM domain as a new class of Zn-containing DNA-binding domain with no similarity to any other DNA-binding domain [PMID: 12682016]. The GCM domain consists of a large and a small domain tethered together by one of the two Zn ions present in the structure. The large and the small domains comprise five- and three-stranded beta-sheets, respectively, with three small helical segments packed against the same side of the two beta-sheets. The GCM domain exercises a novel mode of sequence-specific DNA recognition, where the five-stranded beta-pleated sheet inserts into the major groove of the DNA. Residues protruding from the edge strand of the beta-pleated sheet and the following loop and strand contact the bases and backbone of both DNA strands, providing specificity for its DNA target site.
Hmmscan Out: # of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc

1 3 1.5e-78 1.4e-74 248.6 2.8 1 140 42 181 42 181 0.99

2 3 0.086 7.8e+02 -0.0 0.1 52 78 246 272 238 296 0.75

3 3 0.33 3e+03 -2.0 0.1 26 44 405 423 398 425 0.84

#	of	c-Evalue	i-Evalue	score	bias	hmm coord from	hmm coord to	ali coord from	ali coord to	env coord from	env coord to	acc
1	3	1.5e-78	1.4e-74	248.6	2.8	1	140	42	181	42	181	0.99
2	3	0.086	7.8e+02	-0.0	0.1	52	78	246	272	238	296	0.75
3	3	0.33	3e+03	-2.0	0.1	26	44	405	423	398	425	0.84

Sequence Information

Coding Sequence: ATGTCGAAAGGAACGGTACGCCGCAGAAACGACTCGGATACGTCAGGCATGACAGGATACCCAAGGATCTCAAGACACCAAGAATGGGATATTAACGATTCAAACGTTCCTAGGGTTGTGGAGTATAACAGATTTGAAGAATGGGCGGACGGTCATTGTCGATTGGTGTATCCTCCGGAATCTGAAGAAGGGAAGCGACATTCTTCTGGTTGGGCGATGAGGAATACGAATAACCATAACGTCCATATACTTAAGAAGTCGTGCCTGGGCGTCCTCGTGTGTTCGCGGCGATGTATCTTACCGAACGGCGACTCGGTCCATCTGCGGCCGGCGATCTGCGACAAAGCGAGGAAGAAGCAGCAAGGGAAGCCGTGCCCGAATCGCCTCTGCAACGGGCGGCTCGAGATCCTTCCTTGCAGGGGACACTGCGGTTATCCTGTCACCCATTTCTGGAGACATACGGAGCACGCAATCTTCTTCCAGGCGAAGGGCGTCCACGATCACCTGAGGCCCGAAGCGAAGTCGACTTCAGAAGCGAGGCGGTCGCTAGGAGCAGGGAGACGAGTCAGAGGATTGGCTGTCATGCTCTCCAAAGAAACTTCCCTTAGCAATAAATTATTGTCTCTTCGGGATGTCAAAAGGCCTTCATGTGAAATAGAAACAGCAATTCAGCGCCAAATTATGCCTCCTCCTATATTACAGGATAAAGGTTATTCTTGTTCGTGTCCTCCTTTTGAGTGCGTTTGTAATTGTAATACCGAAGAGAATTCTGAGCTATATCTTTTACCTCCTGAAATCGATTCAAGTCATCAGATGGACTGGTATAGCCAGCAACATCAATATCATAATAATAACAACCCGCAACATCAAAATCATCAACTCCACCATGACGAGAGTACGTCTTTAGAGCCTTATTTTCCTCCTCAGCCACCTCCTCCGGTGCTCCACGTCCAGCAGCATGAAATGTACGAAATGGCCCCTGTAAGCAACGAAATGTACCATCCAGTTTTTCCAAACAACGTCGAATGTGCTGCTTCCGGCTCGCCTCCCACTCTCCTCGACTTGGGCTCAGGAACCATCCATCGGCCCATCAAAGAAGAATGGCCCTTAGATGACCGACAGCAAATGTCTGGAAATTATCCCGAAGAATCTAAATGGTTTCCAAGCGACCCGTTGGCAGATCTGGACGTCCTCACAGGCGACTCTACAATGACTGAATCGTCGTGGACTTCTCAAGATTCCAATTACCAAAATCAGGAGCTCATCAAAAGTTGGTACCCGACTGAACAACCTGTTGTACCTCAAGAGCTCAAAAGTTCATGGAACCTACACCAGGACACCAACCCACAAGAACCTCTCAAGTCCTCCCCTTCGCACGACCCCTTTTTCCAATTCTCTTATTATCAGCCAATTAGTTTGTAA
Protein Sequence: MSKGTVRRRNDSDTSGMTGYPRISRHQEWDINDSNVPRVVEYNRFEEWADGHCRLVYPPESEEGKRHSSGWAMRNTNNHNVHILKKSCLGVLVCSRRCILPNGDSVHLRPAICDKARKKQQGKPCPNRLCNGRLEILPCRGHCGYPVTHFWRHTEHAIFFQAKGVHDHLRPEAKSTSEARRSLGAGRRVRGLAVMLSKETSLSNKLLSLRDVKRPSCEIETAIQRQIMPPPILQDKGYSCSCPPFECVCNCNTEENSELYLLPPEIDSSHQMDWYSQQHQYHNNNNPQHQNHQLHHDESTSLEPYFPPQPPPPVLHVQQHEMYEMAPVSNEMYHPVFPNNVECAASGSPPTLLDLGSGTIHRPIKEEWPLDDRQQMSGNYPEESKWFPSDPLADLDVLTGDSTMTESSWTSQDSNYQNQELIKSWYPTEQPVVPQELKSSWNLHQDTNPQEPLKSSPSHDPFFQFSYYQPISL

Similar Transcription Factors

Sequence clustering based on sequence similarity using MMseqs2

100% Identity: iTF_01108201;
90% Identity: iTF_01108201;
80% Identity: -