Mgen020070.1
Basic Information
- Insect
- Malaya genurostris
- Gene Symbol
- gcm
- Assembly
- GCA_030247185.2
- Location
- CM058072.1:227662701-227670592[-]
Transcription Factor Domain
- TF Family
- GCM
- Domain
- GCM domain
- PFAM
- PF03615
- TF Group
- Beta-Scaffold Factors
- Description
- GCM transcription factors are a family of proteins which contain a GCM motif. The GCM motif is a domain that has been identified in proteins belonging to a family of transcriptional regulators involved in fundamental developmental processes which comprise Drosophila melanogaster GCM and its mammalian homologues [PMID: 8962155, PMID: 9114061, PMID: 9580683, PMID: 10671510]. IN GCM transcription factors the N-terminal moiety contains a DNA-binding domain of 150 residues. Sequence conservation is highest in this GCM domain. In contrast, the C-terminal moiety contains one or two transactivating regions and is only poorly conserved.The GCM motif has been shown to be a DNA binding domain that recognises preferentially the nonpalindromic octamer 5'-ATGCGGGT-3' [PMID: 8962155, PMID: 9114061, PMID: 9580683]. The GCM motif contains many conserved basic amino acid residues, seven cysteine residues, and four histidine residues [PMID: 8962155]. The conserved cysteines are involved in shaping the overall conformation of the domain, in the process of DNA binding and in the redox regulation of DNA binding [PMID: 9580683]. The GCM domain as a new class of Zn-containing DNA-binding domain with no similarity to any other DNA-binding domain [PMID: 12682016]. The GCM domain consists of a large and a small domain tethered together by one of the two Zn ions present in the structure. The large and the small domains comprise five- and three-stranded beta-sheets, respectively, with three small helical segments packed against the same side of the two beta-sheets. The GCM domain exercises a novel mode of sequence-specific DNA recognition, where the five-stranded beta-pleated sheet inserts into the major groove of the DNA. Residues protruding from the edge strand of the beta-pleated sheet and the following loop and strand contact the bases and backbone of both DNA strands, providing specificity for its DNA target site.
- Hmmscan Out
-
# of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc 1 4 1.4e-76 2.9e-72 242.3 6.1 1 140 20 160 20 160 0.98 2 4 0.017 3.7e+02 2.2 0.2 59 100 201 241 193 258 0.73 3 4 0.2 4.4e+03 -1.3 0.0 88 109 366 387 361 409 0.83 4 4 1.5 3.2e+04 -4.0 0.4 15 39 423 448 417 451 0.56
Sequence Information
- Coding Sequence
- ATGTCAGTAGAGTGGGATATCAATGACGCCCACATACCCTTTGTTCAGGACATTAGCTACGATCGATTTCACGAATGGGCCGACGGACATTGTCGCTACGTGTACGTTTCTACCGACGACCAGGCGCGGAAGCACATATCCGGTTGGGCAATGCGGAACACCAACAACCACAATGTAAGCATACTGAAGAAAAGTTGCCTCGGTGTTTTAATCTGCTCGACACGATGCCGTCTTCCTAACGGAAGCCAAATACACCTGCGGCCGGCAATTTGTGATAAAGCCCGCAGGAAACAACAGGGACGAGCTTGTCCCAATCGCAACTGCAAAAGCGGTCGCCTTGAAATATTGGCCTGTCGGGGCCACTGTGGATATCCTGTAACACACTATTGGCGCCATACAGAGAAGGCGATTTTCTTCCAAGCCAAAGGCACCCATGACCATCCGAAGCCAGAGTCCAAAACATCGGGTGAAACTAGGAAACTAGTTGGATCGTGTGGTAAAATCAAATCGAAAAAGTTGTCAGTGCTACTACTGAGGGATGCAGCGTTGGGAAACAAGcttcttcaactaaaaaatGGAAGTAAAGCAGCTTCAACCAGCCAACCATCAGCAGAAATTCTACAACCTCCCCCTTTGATTCCAGATGCAAACCTTGAAAAATCAAAATGCCGACATTGTCTCGAAGCCCAATGTAGTTGCCAAATTATACTTCCTGCCAAAGGCTCGCAGGTTccagaatcgaattttttcttCGTGGATAGTGGCGTCAACTCTTCTCATGGAACCACGTGGCCATGCTCCTCAACTGCTTCAAACAATCACTCGTATGAAACTGCTACTTGTATGGGCCCGTCTGACTATGCTGGTTGTTCTGGGAGTGCtattgaaaatttccaaccaGAAGAGATCTTCCTGCTGGATCAACCATTGAGAAACAGTTCGCAGCAATCGGCCAACATGATCGCTCCAGCTACCACACTGCTAGATCTTGGAAGTGGAgcaatttataaaaaatgtaaTCAAATTGCACCGGATTATTTTCTTGGTAGTAGTAGTGGAAACACATTCACCGAAGCACATCTAGGACAAACTGTTCCTTGTGGAGGTTCGACAGCTTTCGATAAATGTGACGGATATACGAACAGACCTGTTACTACGTCGGGAACTCAAGGTTCTGCGAATTTGTCATACGCTTCGGATATTAACAAAAGTATCAAAAAAGAAAATGCGGCAAAGTTTTGCGATTTAAATTATGCTCAACGGATTCGGTATGACTGTGATAACAAACTAAACTGTAGAAAAACTGGAAAATCCGTCAATACAATTTATacgaacaacaataacaacagcaCATCTTATCAGTATGGAAGCCATCAAGGAATCTACAATCCGTGTGACGATGTCAATTTCAACTTCCCTAGTGAATCCGGTACTTACCAACATCGCTATAATTTAAACTACCTAACTAGTAGCGGATCAAATCAATCGGCTCTCGTATCGACGGCTTTGATGAATGAGGAATGCTACCGATATCCCAACAATGGCTATTCCAAAACTGGTCTAGGTACCAATGACCACGTCTCGCAGCATCAACAAATAGGCGCAGAAGTGCCCATTGCGTCGTACGATTTTTATTAG
- Protein Sequence
- MSVEWDINDAHIPFVQDISYDRFHEWADGHCRYVYVSTDDQARKHISGWAMRNTNNHNVSILKKSCLGVLICSTRCRLPNGSQIHLRPAICDKARRKQQGRACPNRNCKSGRLEILACRGHCGYPVTHYWRHTEKAIFFQAKGTHDHPKPESKTSGETRKLVGSCGKIKSKKLSVLLLRDAALGNKLLQLKNGSKAASTSQPSAEILQPPPLIPDANLEKSKCRHCLEAQCSCQIILPAKGSQVPESNFFFVDSGVNSSHGTTWPCSSTASNNHSYETATCMGPSDYAGCSGSAIENFQPEEIFLLDQPLRNSSQQSANMIAPATTLLDLGSGAIYKKCNQIAPDYFLGSSSGNTFTEAHLGQTVPCGGSTAFDKCDGYTNRPVTTSGTQGSANLSYASDINKSIKKENAAKFCDLNYAQRIRYDCDNKLNCRKTGKSVNTIYTNNNNNSTSYQYGSHQGIYNPCDDVNFNFPSESGTYQHRYNLNYLTSSGSNQSALVSTALMNEECYRYPNNGYSKTGLGTNDHVSQHQQIGAEVPIASYDFY
Similar Transcription Factors
Sequence clustering based on sequence similarity using MMseqs2
- 100% Identity
- -
- 90% Identity
- -
- 80% Identity
- -