Basic Information

Insect: Idaea straminata
Gene Symbol: -
Assembly: GCA_951213275.1
Location: OX577875.1:2424314-2440628[-]

Transcription Factor Domain

TF Family: zf-C2H2
Domain: zf-C2H2 domain
PFAM: PF00096
TF Group: Zinc-Coordinating Group
Description: The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter [1].
Hmmscan Out: # of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc

1 43 0.00073 0.059 14.5 0.9 2 23 94 116 93 116 0.97

2 43 0.067 5.5 8.3 0.2 2 23 138 159 137 159 0.96

3 43 0.0027 0.22 12.7 0.1 1 23 163 185 163 185 0.94

4 43 0.016 1.3 10.3 3.9 1 23 190 212 190 212 0.97

5 43 0.025 2.1 9.6 0.6 2 23 219 241 219 241 0.91

6 43 0.00062 0.05 14.7 0.4 2 23 249 271 248 271 0.97

7 43 0.0056 0.46 11.7 1.8 1 23 277 299 277 299 0.98

8 43 0.05 4 8.7 1.2 1 23 305 327 305 327 0.98

9 43 2.2e-06 0.00018 22.4 0.8 1 23 333 356 333 356 0.97

10 43 1 82 4.6 1.0 1 17 398 414 398 421 0.81

11 43 0.44 36 5.7 0.4 2 23 445 467 444 467 0.91

12 43 0.0044 0.36 12.0 0.2 1 23 489 513 489 513 0.97

13 43 0.0019 0.15 13.2 0.1 1 23 517 539 517 539 0.97

14 43 0.00094 0.076 14.1 3.0 1 23 582 605 582 605 0.98

15 43 0.0014 0.12 13.5 5.4 1 23 611 633 611 633 0.98

16 43 1.6e-06 0.00013 22.8 2.1 1 23 639 661 639 661 0.98

17 43 3.3e-05 0.0027 18.7 0.3 1 23 667 690 667 690 0.98

18 43 0.016 1.3 10.3 2.1 1 23 743 766 743 766 0.95

19 43 0.0029 0.24 12.6 1.0 2 23 837 858 836 858 0.97

20 43 0.12 9.6 7.5 1.0 1 23 862 884 862 884 0.98

21 43 0.011 0.88 10.8 1.9 2 23 899 921 898 921 0.97

22 43 0.041 3.3 9.0 6.4 1 23 927 950 927 950 0.97

23 43 0.31 26 6.2 5.5 1 23 956 978 956 978 0.97

24 43 2.3e-06 0.00019 22.3 0.8 1 23 984 1006 984 1006 0.99

25 43 0.025 2 9.6 1.0 1 13 1012 1024 1012 1025 0.93

26 43 0.006 0.49 11.6 1.2 1 23 1063 1086 1063 1086 0.93

27 43 0.041 3.3 9.0 0.4 2 23 1156 1177 1155 1177 0.97

28 43 0.00057 0.047 14.8 0.5 1 23 1181 1203 1181 1203 0.97

29 43 0.023 1.9 9.7 0.2 2 23 1217 1239 1217 1239 0.94

30 43 4.9e-05 0.004 18.2 1.0 2 23 1246 1268 1245 1268 0.96

31 43 0.011 0.92 10.7 3.2 1 23 1274 1296 1274 1296 0.98

32 43 1.4e-06 0.00011 23.1 1.5 1 23 1302 1324 1302 1324 0.99

33 43 4.5e-06 0.00037 21.4 1.2 1 23 1330 1353 1330 1353 0.98

34 43 0.2 16 6.8 0.4 2 23 1444 1466 1443 1466 0.94

35 43 0.12 9.5 7.5 0.1 2 20 1489 1507 1488 1509 0.93

36 43 1.1 93 4.4 1.0 1 22 1535 1556 1535 1558 0.86

37 43 8.8 7.2e+02 1.6 0.2 2 23 1580 1602 1579 1602 0.92

38 43 0.00049 0.04 15.0 0.6 2 23 1625 1646 1624 1646 0.97

39 43 0.57 46 5.4 0.2 1 21 1650 1670 1650 1673 0.94

40 43 0.00066 0.054 14.6 1.6 2 23 1692 1714 1691 1714 0.94

41 43 4.3e-05 0.0035 18.3 2.6 1 23 1720 1743 1720 1743 0.96

42 43 0.0047 0.38 11.9 3.1 1 20 1749 1768 1749 1771 0.93

43 43 0.00011 0.0087 17.1 0.6 1 23 1777 1799 1777 1799 0.96

#	of	c-Evalue	i-Evalue	score	bias	hmm coord from	hmm coord to	ali coord from	ali coord to	env coord from	env coord to	acc
1	43	0.00073	0.059	14.5	0.9	2	23	94	116	93	116	0.97
2	43	0.067	5.5	8.3	0.2	2	23	138	159	137	159	0.96
3	43	0.0027	0.22	12.7	0.1	1	23	163	185	163	185	0.94
4	43	0.016	1.3	10.3	3.9	1	23	190	212	190	212	0.97
5	43	0.025	2.1	9.6	0.6	2	23	219	241	219	241	0.91
6	43	0.00062	0.05	14.7	0.4	2	23	249	271	248	271	0.97
7	43	0.0056	0.46	11.7	1.8	1	23	277	299	277	299	0.98
8	43	0.05	4	8.7	1.2	1	23	305	327	305	327	0.98
9	43	2.2e-06	0.00018	22.4	0.8	1	23	333	356	333	356	0.97
10	43	1	82	4.6	1.0	1	17	398	414	398	421	0.81
11	43	0.44	36	5.7	0.4	2	23	445	467	444	467	0.91
12	43	0.0044	0.36	12.0	0.2	1	23	489	513	489	513	0.97
13	43	0.0019	0.15	13.2	0.1	1	23	517	539	517	539	0.97
14	43	0.00094	0.076	14.1	3.0	1	23	582	605	582	605	0.98
15	43	0.0014	0.12	13.5	5.4	1	23	611	633	611	633	0.98
16	43	1.6e-06	0.00013	22.8	2.1	1	23	639	661	639	661	0.98
17	43	3.3e-05	0.0027	18.7	0.3	1	23	667	690	667	690	0.98
18	43	0.016	1.3	10.3	2.1	1	23	743	766	743	766	0.95
19	43	0.0029	0.24	12.6	1.0	2	23	837	858	836	858	0.97
20	43	0.12	9.6	7.5	1.0	1	23	862	884	862	884	0.98
21	43	0.011	0.88	10.8	1.9	2	23	899	921	898	921	0.97
22	43	0.041	3.3	9.0	6.4	1	23	927	950	927	950	0.97
23	43	0.31	26	6.2	5.5	1	23	956	978	956	978	0.97
24	43	2.3e-06	0.00019	22.3	0.8	1	23	984	1006	984	1006	0.99
25	43	0.025	2	9.6	1.0	1	13	1012	1024	1012	1025	0.93
26	43	0.006	0.49	11.6	1.2	1	23	1063	1086	1063	1086	0.93
27	43	0.041	3.3	9.0	0.4	2	23	1156	1177	1155	1177	0.97
28	43	0.00057	0.047	14.8	0.5	1	23	1181	1203	1181	1203	0.97
29	43	0.023	1.9	9.7	0.2	2	23	1217	1239	1217	1239	0.94
30	43	4.9e-05	0.004	18.2	1.0	2	23	1246	1268	1245	1268	0.96
31	43	0.011	0.92	10.7	3.2	1	23	1274	1296	1274	1296	0.98
32	43	1.4e-06	0.00011	23.1	1.5	1	23	1302	1324	1302	1324	0.99
33	43	4.5e-06	0.00037	21.4	1.2	1	23	1330	1353	1330	1353	0.98
34	43	0.2	16	6.8	0.4	2	23	1444	1466	1443	1466	0.94
35	43	0.12	9.5	7.5	0.1	2	20	1489	1507	1488	1509	0.93
36	43	1.1	93	4.4	1.0	1	22	1535	1556	1535	1558	0.86
37	43	8.8	7.2e+02	1.6	0.2	2	23	1580	1602	1579	1602	0.92
38	43	0.00049	0.04	15.0	0.6	2	23	1625	1646	1624	1646	0.97
39	43	0.57	46	5.4	0.2	1	21	1650	1670	1650	1673	0.94
40	43	0.00066	0.054	14.6	1.6	2	23	1692	1714	1691	1714	0.94
41	43	4.3e-05	0.0035	18.3	2.6	1	23	1720	1743	1720	1743	0.96
42	43	0.0047	0.38	11.9	3.1	1	20	1749	1768	1749	1771	0.93
43	43	0.00011	0.0087	17.1	0.6	1	23	1777	1799	1777	1799	0.96

Sequence Information

Coding Sequence: ATGGAGTCTAACATCGCCAATAACAGGAAAGCGAAAGGTAAACCAAGCGCAGAGTTATTGATGGCCAGGGAAAATCTCttagcaatatttaattattcaaatGCTACTCCGATTCAATCCCAACACGCACAGAGCTTCTTCTGCTGCTATTGTAATTTTGGACACCCTAAACCCAGCCAACTGAAAACGCACGTCTTAGAATCACACATTCGATCCACGtctttcaaaagaaaaatagctATAAAAGATCTTACCGTTAAATTAGATATAACTGATCTAAAATGCACTGTTTGCGAAAAAAGCGTCGTACATCTAGAAGAATTGATGAGTCATATCAAAACTAAACATAATGTTGAAttaaatgatattaaaaatcCATTGATACCGTTCAAATTTGATGATGAGAAGCTGTCCTGCTATATTTGTTCAGCGGAATTCACAAATTTTGTGAAATTAACCGGCCATCTAAACGTGCATTATCAGAATCATATATGCGAAGTCTGCGGGGCAGGATTCATATCCCGCGGGCAGTTACAGACCCATGGCCAGGCTCACCTCACTGGAACTTTCAACTGCTCAAAATGCTCAAAGACTTTCGACACTGAGCGTAATAAAAAAAGCCATGAGAAATCTCACGATGGTAAATTACGTCGGAAATGTTTTGAATGCGACGAACGCTTTAGTACTCAGATTGCGAAAGCAAAGCATATGGTGGAGAAACACGGTGCACCAGAACTGACTGTCAAATGTCAGTATTGCAGCAAGGTGTTCGGTTACTACGAGAATATGATGGTTCACATTAAGAGAGTGCATTTAAGGGAGCGTAATCACGAATGCCCATATTGCGATATGAAATTCTTCACTATAGAAGCGAAGAAAACCCATATACAAACGCACACGGGCGAAAAGAAATTCAAATGTGATATCTGTTACAATATGTATAAAACTCAGACAATTTTAAGAGAACATCTAAAGATTCATTTCGATATACGCAGATTTAAGTGTGAAGTTTGCGATAAGAGCTTCATTCAAAAGTCTGGGTTAAAGGGACATATGTCAAGCAAACATGGATTACGCGAGCCTACCGATCGCGCGAAGATTCTAACCAGGAAGAGACAGAAAAAGGATACACTACAGAAGATAGTGGCTTATTCTAACGCAACACCCGTACTTAAATGCAAAGGAATGTTCTACTATTGTTCGTATTGCGATGAGAGATTCCTTAACCCAGAAAAACTAAAGTTTCACTCATTGGAAAGTCACCAGAatgattttatgaaattgaatTGTTTAGCCGGAGAATATATCATCAAGATAGACATCACTGATCTTACCTGTAAGCTTTGCAGTACTTTCGTCTCATCAATGCAAGAGTTAATCAAACATCTAACTGATGAACATGAGATAAAGTTTGAACCGATCGATTTTACTAAGTTCTTGATTCCTTTTAAGTTTAACGGCATGTTTCGTTGCGGGGTCGATACTTGCGACGCCTCTTTTAATACTTACCAAGGTGTTATTAAACATATGAATACCCATCTAGCAAATTACGTTTGCGAAATTTGCAACAACTCTTATTCTACTAAAGTGGGTTTAGCGTTGCACGTCAAAGgtcatactggtgaaaaacccaAGAAGGCATCACGAGTTAAGAGAATAAAATGCCCGCATTGCGATTTCGTTTCCAAACATAGTCATGGCCATGGCATTCATATGAAAGAAATGCACGGTTTGGCGACGAATTTCAAATGCACGTCGTGCGATCGCGAATTTCAGAACAGCATGCACTTACGCGTACATGTAAAGCGCGACCACTTAATGGAAAGGAACCATAAGTGTCCGTTTTGCGAGAAGAGCTTTTTCTACAGGACGAAATTGAACAATCATTTAACAACTCATACGGGGGAAAGACGATTTCAATGTCTTATCTGCGATAAaagatttaaaactaaatataacttGACGATCCATAATCGAATACATACCAACGACAAGCGATATAAGTGTAGAGTTTGTGGTAAAGCATATGCTCAGAGGAGCTGGGTTAATGCTCATATTAAATCCAAACATGGCGAAGTGGAAATTTTAGAACATTATTGCATTGAGGAAGATATGCAATgcCATCGATTGAAGTGGATATCGCTCAAACGCAAGAAAAAGGATACAGTCAAGCTGTTGGTGATGTACACAAATGCCAACCCGGTCCAAAAGATAAAAGAAATGTTTTATTACTGTAGCTATTGCGATGCCCGGTACCATAACCCAGTAAAACTAAAAGTTCACACACAAGAAAAGCATAAATACGAATTCCACAAGTACTTTTTCTCACCATCTAAAGAGTTCTTAGTCAAATTCGACATAACAAACTTAACTTGCGACCTTTGTGATGCCGAAGTGGATTCTGTCGAAGAAATTATCAAACATTTAACAAGTCAACACGAAAAGAAGTTCCCTATTGTAGATGTCAACAGTTTtctaatttcatttaaatttatagACAGTATGCTCCGTTGTGCCTATTGCGATGCCTCTTTTAATACCTTCCGAAGACTCCAGATGCATATAAACAAGCATATGGCTAATTATACCTGTGAATATTGCCAAGCCACTTACAAGGAACGGAAAATATTCATTCGCCATGTAGAAACTCATACAGACAAAGTAAGAGTAAAGCAAAAGAGGTTATACGCCTCGAAATGTCAGCATTGCGATATGAGCTTCAACtcttatgaaacaaaaatggATCATTTAAGAGTAGCTCATGGATTAGCAGTCACTCACAAATGTAACTCATGCGATCGCGTATTCACTTGTAATACCACTTTACGACGCCATGTCAAAAGAGATCACTTATTAGAAAGGAACTTCAAATGTTCTTTCTGCGAGAAAAGCTTTTTCTGCAAAGTGAAGTTAACTAACCATCTTACAGTGCATACGGGAGAACAGAACTTCCAGTGTGAAGTTTGCAACAAGCGGGTAAAGACTAAAGGGACTTTGCGGGTCCATATGCGAATACATACGGATGGCAAGCAGTATAAATGTGAGAAGTGTGGGAAAGCATTTGCTCAGAAGAAAATACCTAAACAGAAGAATACTCAAGCAACAAATCTTCAAAAAAAGGATACAGTCACGAAGATACTCAGCTGGTCTAACGCAACCCCAGTACGAAAATCTTCAGGGTTATTTTACCACTGTTCTTACTGCGACGCCAAGTTCCCTAACCCTATCAAACTAAAAGAACATACTGAGGAATTCCACAAAAatgagatagataaattcttcatCAGCCCGAGTCATAGTTTTAAGATTAATATAGATGTCACCAATTTGACTTGTCGAATCTGCAGTGGTATCCAATACTCTATTAAGGAACTTATAACTCATCTAATACAAGAACATGATAAGAAATTAGACTGTATTGATGTTAAAAAATTCGTTATGCCATTCAGATATGACGCTATGCTTCGATGTGTGTACTGCGAAGCTtcttttaatagttttaacatACTCCAAGCTCACATGAATAAACACTTAGCCAATTACTTCTGCGATATTTGCGATTCTACATACTCATCCCTTGAAGGTCTAAAACAACATATAAAGAatcatagtaaaaaaatatccgaGAAAAAGAGAACTCGTAATAACGTATGTCCACAGTGTGGTCTGAACTTCGCTACTAACACTTTAAAAGTTAAACACCTAAAAGGCGAACATGGTTTGGAGTTAAAGAAGCAATGTAATTCTTGCGAACGCTCGTTTACAAACAGCTCTCAACTTTGGGTGCATGTCAAAAGAGACCATTTAATGGAAAGAAGGTTTCAATGTAATTTATGCGATAAGTCGTTTTTTTGCAAAGAAAAGTTAGTAAACCACATAACTGTGCATACAGGAGAGCAGAAGTACCAGTGTGATGTTTGTTCTAAAGGGTTTAAATACAAGTCTAATTTGACAGTTCATAAACGTATTCATACCAATGACAAACGGTTTAAATGTCATATATGTGGGTTGGCGTACGCGCAGAACAGCTGGCTGAGGATGCATATGAAATCTAGGCATAATGAACATTGTTTCTTTATAGGCAATAAAGATAGCCTGAATCCCTATAAGCTGGCTCTACAGAAGACGGACCTACTGCAGCAAATCCTTGCGTCGTCGAACGCTAATCCTGTCAAATTCAATGGACAGTTTTACCGCTGTTACTACTGTCTTGAACAATTCCTGAGCCCGAAGAAGCTGAAGTTTCACGCTGAAGAAGATCACGCGGATGACACCATTACTCACAAACAGAGAGATAATATGGTTGTAAAACTGGACGTAACAAATCTGACTTGCAAACTATGTGGGAACTGTGTAAACTCCATCCAGGACTTAATCGAACATTTAAAGGAAGTTCACGAGAAGAAATTTCAAACTGAAGATGTATCCAAGTTAATTTTACCATTTATTTACGAAGATGGCTTAAAATGTGCCATCTGTGGTATTAGTTTATGCAATAAAGATAGCGTGAATTGGCATAAGCTGGCTCTGCAGAAAACGGACTTACTGCAGCAACTGCTTGCGTCATCAAACGCTACTCCTGTCAAATTCAATGGACAGTTTTACCGCTGTGGCTACTGCTTAGAACGGTTTATGAGCCCGAAGAAGCTGAAGTTTCACACTGAAGAAGACCACGCGAATGACACCATTACTCATAAGAAGAGAGAGAAGGTTGTAAAACTGGATGTAACAAATCTGACTTGCAAGCTATGCAAGAATATTATAAACTCAATCCAGGACTTAATTAGTCATTTAAAAGATGAACACGACAAGATATTCCAAACAGAAGATATATCTTCCTTCATTATACCATTTATTTACGAAGATAGCTTAAAATGCACTGTCTGTAATCTCAAATTAAGTAATTCTCGAAGTCTAGTTGTCCATATGAATACGCATTTCGGGAATTTCGTCTGCGATATTTGCAGTGCGAGTTATATGATACGGGACGGATTAGAGGCACATTTAAAATGTATTCATAAGAAAGAACAACGCGTTACGAATACCAAAAAAAGGGGTTATAATAGGAACTGTAAGTGTCCACATTGCGAGCTAGTATTCACTAGACTGCCGGCATTAAGACTGCATCTGAAAGAAGAGCATGGTGACGCCAAACCTTTCGCTTGTCAAACTTGTGATCGCACTTACACCAAATCCAGTCACTTATGGAGCCACATAAAGAGAGATCACTTAAAAGAACGGAAGCATAAGTGCAATTTTTGCGAGAAAGGTTTCTTTTCTAAGGACAAATTAACGAAACATTTGCCCGTACACACTGGGGAAGTGAAATACTTGTGCGATGTTTGTCCTAAAGCTTTTAAGATGCAATACAGTTTGTCCGTTCATAAAAAGACGCATGTTAATGATAAACGGGTCAAATAA
Protein Sequence: MESNIANNRKAKGKPSAELLMARENLLAIFNYSNATPIQSQHAQSFFCCYCNFGHPKPSQLKTHVLESHIRSTSFKRKIAIKDLTVKLDITDLKCTVCEKSVVHLEELMSHIKTKHNVELNDIKNPLIPFKFDDEKLSCYICSAEFTNFVKLTGHLNVHYQNHICEVCGAGFISRGQLQTHGQAHLTGTFNCSKCSKTFDTERNKKSHEKSHDGKLRRKCFECDERFSTQIAKAKHMVEKHGAPELTVKCQYCSKVFGYYENMMVHIKRVHLRERNHECPYCDMKFFTIEAKKTHIQTHTGEKKFKCDICYNMYKTQTILREHLKIHFDIRRFKCEVCDKSFIQKSGLKGHMSSKHGLREPTDRAKILTRKRQKKDTLQKIVAYSNATPVLKCKGMFYYCSYCDERFLNPEKLKFHSLESHQNDFMKLNCLAGEYIIKIDITDLTCKLCSTFVSSMQELIKHLTDEHEIKFEPIDFTKFLIPFKFNGMFRCGVDTCDASFNTYQGVIKHMNTHLANYVCEICNNSYSTKVGLALHVKGHTGEKPKKASRVKRIKCPHCDFVSKHSHGHGIHMKEMHGLATNFKCTSCDREFQNSMHLRVHVKRDHLMERNHKCPFCEKSFFYRTKLNNHLTTHTGERRFQCLICDKRFKTKYNLTIHNRIHTNDKRYKCRVCGKAYAQRSWVNAHIKSKHGEVEILEHYCIEEDMQCHRLKWISLKRKKKDTVKLLVMYTNANPVQKIKEMFYYCSYCDARYHNPVKLKVHTQEKHKYEFHKYFFSPSKEFLVKFDITNLTCDLCDAEVDSVEEIIKHLTSQHEKKFPIVDVNSFLISFKFIDSMLRCAYCDASFNTFRRLQMHINKHMANYTCEYCQATYKERKIFIRHVETHTDKVRVKQKRLYASKCQHCDMSFNSYETKMDHLRVAHGLAVTHKCNSCDRVFTCNTTLRRHVKRDHLLERNFKCSFCEKSFFCKVKLTNHLTVHTGEQNFQCEVCNKRVKTKGTLRVHMRIHTDGKQYKCEKCGKAFAQKKIPKQKNTQATNLQKKDTVTKILSWSNATPVRKSSGLFYHCSYCDAKFPNPIKLKEHTEEFHKNEIDKFFISPSHSFKINIDVTNLTCRICSGIQYSIKELITHLIQEHDKKLDCIDVKKFVMPFRYDAMLRCVYCEASFNSFNILQAHMNKHLANYFCDICDSTYSSLEGLKQHIKNHSKKISEKKRTRNNVCPQCGLNFATNTLKVKHLKGEHGLELKKQCNSCERSFTNSSQLWVHVKRDHLMERRFQCNLCDKSFFCKEKLVNHITVHTGEQKYQCDVCSKGFKYKSNLTVHKRIHTNDKRFKCHICGLAYAQNSWLRMHMKSRHNEHCFFIGNKDSLNPYKLALQKTDLLQQILASSNANPVKFNGQFYRCYYCLEQFLSPKKLKFHAEEDHADDTITHKQRDNMVVKLDVTNLTCKLCGNCVNSIQDLIEHLKEVHEKKFQTEDVSKLILPFIYEDGLKCAICGISLCNKDSVNWHKLALQKTDLLQQLLASSNATPVKFNGQFYRCGYCLERFMSPKKLKFHTEEDHANDTITHKKREKVVKLDVTNLTCKLCKNIINSIQDLISHLKDEHDKIFQTEDISSFIIPFIYEDSLKCTVCNLKLSNSRSLVVHMNTHFGNFVCDICSASYMIRDGLEAHLKCIHKKEQRVTNTKKRGYNRNCKCPHCELVFTRLPALRLHLKEEHGDAKPFACQTCDRTYTKSSHLWSHIKRDHLKERKHKCNFCEKGFFSKDKLTKHLPVHTGEVKYLCDVCPKAFKMQYSLSVHKKTHVNDKRVK

Similar Transcription Factors

Sequence clustering based on sequence similarity using MMseqs2

100% Identity: -
90% Identity: -
80% Identity: -