Basic Information

Insect: Flexamia atlantica
Gene Symbol: gcm
Assembly: GCA_035578135.1
Location: JAQJVK010000004.1:38788447-38807720[+]

Transcription Factor Domain

TF Family: GCM
Domain: GCM domain
PFAM: PF03615
TF Group: Beta-Scaffold Factors
Description: GCM transcription factors are a family of proteins which contain a GCM motif. The GCM motif is a domain that has been identified in proteins belonging to a family of transcriptional regulators involved in fundamental developmental processes which comprise Drosophila melanogaster GCM and its mammalian homologues [PMID: 8962155, PMID: 9114061, PMID: 9580683, PMID: 10671510]. IN GCM transcription factors the N-terminal moiety contains a DNA-binding domain of 150 residues. Sequence conservation is highest in this GCM domain. In contrast, the C-terminal moiety contains one or two transactivating regions and is only poorly conserved.The GCM motif has been shown to be a DNA binding domain that recognises preferentially the nonpalindromic octamer 5'-ATGCGGGT-3' [PMID: 8962155, PMID: 9114061, PMID: 9580683]. The GCM motif contains many conserved basic amino acid residues, seven cysteine residues, and four histidine residues [PMID: 8962155]. The conserved cysteines are involved in shaping the overall conformation of the domain, in the process of DNA binding and in the redox regulation of DNA binding [PMID: 9580683]. The GCM domain as a new class of Zn-containing DNA-binding domain with no similarity to any other DNA-binding domain [PMID: 12682016]. The GCM domain consists of a large and a small domain tethered together by one of the two Zn ions present in the structure. The large and the small domains comprise five- and three-stranded beta-sheets, respectively, with three small helical segments packed against the same side of the two beta-sheets. The GCM domain exercises a novel mode of sequence-specific DNA recognition, where the five-stranded beta-pleated sheet inserts into the major groove of the DNA. Residues protruding from the edge strand of the beta-pleated sheet and the following loop and strand contact the bases and backbone of both DNA strands, providing specificity for its DNA target site.
Hmmscan Out: # of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc

1 5 3.3e-16 1.6e-11 46.7 0.8 1 40 229 268 229 270 0.95

2 5 1.9e-17 8.7e-13 50.7 0.9 1 40 271 310 271 312 0.95

3 5 1.9e-17 8.7e-13 50.7 0.9 1 40 313 352 313 354 0.95

4 5 1.9e-17 8.7e-13 50.7 0.9 1 40 355 394 355 396 0.95

5 5 2.6e-78 1.2e-73 247.9 4.6 1 140 397 536 397 536 0.99

#	of	c-Evalue	i-Evalue	score	bias	hmm coord from	hmm coord to	ali coord from	ali coord to	env coord from	env coord to	acc
1	5	3.3e-16	1.6e-11	46.7	0.8	1	40	229	268	229	270	0.95
2	5	1.9e-17	8.7e-13	50.7	0.9	1	40	271	310	271	312	0.95
3	5	1.9e-17	8.7e-13	50.7	0.9	1	40	313	352	313	354	0.95
4	5	1.9e-17	8.7e-13	50.7	0.9	1	40	355	394	355	396	0.95
5	5	2.6e-78	1.2e-73	247.9	4.6	1	140	397	536	397	536	0.99

Sequence Information

Coding Sequence: atGGCCAACAATGAGCGATACGGGAGTAACAAGCAACAGTTTCGCCGACAATGGCCGTTGAGAGGGCGGCTGAGCCTGCAGAGCGCACTTATCGGACAAAACGTCCAATTTAAGATCGTGAGAGAGCCCGGGTCGCCGGCCATGTCAGTGTACGGCTACAGAGGGTCGACCTCGcggctgttgttgttgttgttgttgttgtgcaGGAGAGAGCCCGGGTCGCCGGCCATGTCAGTGTACGGCTACAGAGGGAGAGAGCCCGGGTCGCCGGCCATGTCAGTGTACGGCTACAGAGGGTCGACCTCGcggctgttgttgttgttgttgttgttgttgttgtgcaGGAGAGAGCCCGGGTCGCCGGCCATGTCAGTGTACGGCTACAGATGGAGAGAGCCCGGGTCGCCGGTCATGTCAGTGTACGGCTACAGAGGGAGAGAGCCCGGGTCGCCGGCCATGTCAGTGTACGGCTACAGAGGGTCGACCTCGcggctgttgttgttgttgttgtctgAATGTTTGTTGTTGTGCAGGAGAGAGCCCGGGTCGCCGGCCATGTCAGTGTGCGGCTACAGAGGGAGAGAGCCCGGGTCGCCGGCCATGTCAGTGTACGGCTACAGAGGGTCGACCTCGCGGCTGGCGGACTGGGACATCAACGACTCCAGCGTGCCCCGGGTCAGCGAGTTCAACCAGTGGCAGGAGTGGGCGGACGGGCACTGCCGCCTGGTGTACCCGCCGTCCAGCGAGGAGGCCAAGCGCCACGCCAGCGGCTGGGCCATGCGCAacaccaacaacaacaacGTGAGCGAGTTCAACCAGTGGCAGGAGTGGGCGGACGGGCACTGCCGCCTGGTGTACCCGCCGTCCAGCGAGGAGGCCAAGCGCCACGCCAGCGGCTGGGCCATGCGCAACACCAACAACCACAACGTGAGCGAGTTCAACCAGTGGCAGGAGTGGGCGGACGGGCACTGCCGCCTGGTGTACCCACCGTCCAGCGAGGAGGCCAAGCGCCACGCCAGCGGCTGGGCCATGCGCAACACCAACAACCACAACGTGAGCGAGTTCAACCAGTGGCAGGAGTGGGCGGACGGGCACTGCCGCCTGGTGTACCCGCCGTCCAGCGAGGAGGCCAAGCGCCACGCCAGCGGCTGGGCCATGCGCAACACCAACAACCACAACGTCAGCGAGTTCAACCAGTGGCAGGAGTGGGCGGACGGGCACTGCCGCCTGGTGTACCCACCGTCCAGCGAGGAGGCCAAGCGCCACGCCAGCGGCTGGGCCATGCGCAACACCAACAACCACAACGTCCACATCCTCAAGAAGTCCTGCCTGGGCGTCCTGGTGTGCAGTCGGCGCTGTGTCCTGCCTAATGGGGACTCTGTGCATCTGCGGCCCGCGATATGTGACAAAGCGAGGAAGAAACAGCAAGGCAAGCCGTGCCCCAACCGGCAGTGTACCGGACGGTTGGAGATCCTACCCTGCCGCGGCCACTGCGGCTACCCCGTCACCCACTTCTGGCGACACACCGAGCACGCCATCTTCTTCCAGGCGAAGGGGGTGCACGACCACCTGCGGCCCGAGGCCAAGTCGACGAGCGAGGCGCGCCGCAGCCTGGGCGCCGGCCGCCGGGTCCGCGGGCTCGCCGTGCTGCTGGCCCGCGAGGCCTCCCTCGGCAGCAAGTTGCTAACTCTGAGGGATTCCAAGAGGCCTTCTTGTGATCTCGATGAGTCTTCGGGCCCTCGGCAGTTGTTACCCCCTCCCCTCATATCGGACACCGATAAAGCAGGGTACAGCTGCTCATGCCCGCCCTTCGAGTGTGTCTGCTCCTCGCGCGTCTGCCCCTCGCAGATGCAGAGCTCCTGGGACGCCTACTGGCCGCAGGAGACGCCAGCCCTCCCGCAGACTCCCGAACACTACTTCGAAGGCAACTCCTCGACCCCCCTCTCCTACGACCTCAACCTCTGCAACGACCTCTTCCAACCGGAGGAGATCTTCCAGCTGGACCAACCGCTTCGGACGTCCGAGATGCCTGTCACGACTGAAGCCACGTCCAAGTCACCCACCACTCTTTTGGATCTGGGTAGTGGAACCATCCACCGGAGCAGCCTGAAGTCAGAACCGGAGCCCTACTGGTCGCTGGCCGGAGCGGGCGGAGCAGCCATGAGTGTCGAGGATAGCAACAGCAGCTGCCAACTCCCCCAGTGTTCCCCCGAGTCCATCTCCCACACCATGGAGGACACCTCCGCCTGCTCAGTCACCCGATCTCACTACTCCTCCGACTACATGCAGCGCGGTGCTCTAGGGCTCATGAACTTTCACTTCGACGAGAAAGACAGTTACAAGTTCCACGAGGGAGACCAGTCTAGGATGTATTACGAGGACGCGAGGTACCTGAATTGTTCCGAACCTCTCATGGTCCCTGAGGAGTACCAAACGCCCACGCACGAGTCCCGACTCATGGTTCCTTACGCGGAACCTGGTGTATCGGAGTGCTACGCTAGACCCCCTCAGTTCCCTGACCAAAGCAATAGCTCGCAAGAAGATTGCCTCGCTTTTCCCCATGCGAGTTCGCTCGGTGATACGAATTTTTTCCAGTcgtttcaatttcaaaacaacaactacTTCCAGCACCTACCGCACCATTAG
Protein Sequence: MANNERYGSNKQQFRRQWPLRGRLSLQSALIGQNVQFKIVREPGSPAMSVYGYRGSTSRLLLLLLLLCRREPGSPAMSVYGYRGREPGSPAMSVYGYRGSTSRLLLLLLLLLLCRREPGSPAMSVYGYRWREPGSPVMSVYGYRGREPGSPAMSVYGYRGSTSRLLLLLLSECLLLCRREPGSPAMSVCGYRGREPGSPAMSVYGYRGSTSRLADWDINDSSVPRVSEFNQWQEWADGHCRLVYPPSSEEAKRHASGWAMRNTNNNNVSEFNQWQEWADGHCRLVYPPSSEEAKRHASGWAMRNTNNHNVSEFNQWQEWADGHCRLVYPPSSEEAKRHASGWAMRNTNNHNVSEFNQWQEWADGHCRLVYPPSSEEAKRHASGWAMRNTNNHNVSEFNQWQEWADGHCRLVYPPSSEEAKRHASGWAMRNTNNHNVHILKKSCLGVLVCSRRCVLPNGDSVHLRPAICDKARKKQQGKPCPNRQCTGRLEILPCRGHCGYPVTHFWRHTEHAIFFQAKGVHDHLRPEAKSTSEARRSLGAGRRVRGLAVLLAREASLGSKLLTLRDSKRPSCDLDESSGPRQLLPPPLISDTDKAGYSCSCPPFECVCSSRVCPSQMQSSWDAYWPQETPALPQTPEHYFEGNSSTPLSYDLNLCNDLFQPEEIFQLDQPLRTSEMPVTTEATSKSPTTLLDLGSGTIHRSSLKSEPEPYWSLAGAGGAAMSVEDSNSSCQLPQCSPESISHTMEDTSACSVTRSHYSSDYMQRGALGLMNFHFDEKDSYKFHEGDQSRMYYEDARYLNCSEPLMVPEEYQTPTHESRLMVPYAEPGVSECYARPPQFPDQSNSSQEDCLAFPHASSLGDTNFFQSFQFQNNNYFQHLPHH

Similar Transcription Factors

Sequence clustering based on sequence similarity using MMseqs2

100% Identity: -
90% Identity: -
80% Identity: -