Emer009163.2


Basic Information
Transcription Factor Domain
Sequence Information
Similer Transcription Factors

Basic Information

Insect
Eudonia mercurella
Gene Symbol
gcm
Assembly
GCA_963082485.1
Location
OY720024.1:8132244-8137373[+]

Transcription Factor Domain

TF Family
GCM
Domain
GCM domain
PFAM
PF03615
TF Group
Beta-Scaffold Factors
Description
GCM transcription factors are a family of proteins which contain a GCM motif. The GCM motif is a domain that has been identified in proteins belonging to a family of transcriptional regulators involved in fundamental developmental processes which comprise Drosophila melanogaster GCM and its mammalian homologues [PMID: 8962155, PMID: 9114061, PMID: 9580683, PMID: 10671510]. IN GCM transcription factors the N-terminal moiety contains a DNA-binding domain of 150 residues. Sequence conservation is highest in this GCM domain. In contrast, the C-terminal moiety contains one or two transactivating regions and is only poorly conserved.The GCM motif has been shown to be a DNA binding domain that recognises preferentially the nonpalindromic octamer 5'-ATGCGGGT-3' [PMID: 8962155, PMID: 9114061, PMID: 9580683]. The GCM motif contains many conserved basic amino acid residues, seven cysteine residues, and four histidine residues [PMID: 8962155]. The conserved cysteines are involved in shaping the overall conformation of the domain, in the process of DNA binding and in the redox regulation of DNA binding [PMID: 9580683]. The GCM domain as a new class of Zn-containing DNA-binding domain with no similarity to any other DNA-binding domain [PMID: 12682016]. The GCM domain consists of a large and a small domain tethered together by one of the two Zn ions present in the structure. The large and the small domains comprise five- and three-stranded beta-sheets, respectively, with three small helical segments packed against the same side of the two beta-sheets. The GCM domain exercises a novel mode of sequence-specific DNA recognition, where the five-stranded beta-pleated sheet inserts into the major groove of the DNA. Residues protruding from the edge strand of the beta-pleated sheet and the following loop and strand contact the bases and backbone of both DNA strands, providing specificity for its DNA target site.
Hmmscan Out
# of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc
1 1 2.8e-76 6e-72 241.3 4.5 1 140 136 276 136 276 0.98

Sequence Information

Coding Sequence
ATGTATCCACTGTGGCCCTTGGTGTGGTCTTTTGCTCATAGCACGTATAATTTCTACAGGAATattatcaacccatctttgggttccgtagagttgtcgtgcaAAGCTGATGCAAGTAACGCCTGGTTATCCGCCTGTCAAAATTCTATCTTACCATTATTACCACAATCTCAGAGGCAATGGGACGAGCCACTGtgcaagcaagttctggaggggCTTATATCCACTTCCAGTGATATGCCAGATATCGCTCGTCTTACCTACTGCCGTCTCCCAGAGAGTCAGGCTACTGGCTCCGGCATTACCAtctgccaatgtcgTACCAGCAGCCGCGCGGACATGTCAGAAGGGCCAGCCACACCTGAATGGGACATTAACGACGCGGTGGTACCACGGGTTAGCAGTTTCGACACCTTTAGCGAGTGGTGCGACGGCCACGTGAGGCGAGTGTACCCGCCAGGCTGTGAGGAGGCCCGCAGACACGCCTCAGGCTGGGCGATGAGGAACACCAACAACCATAACGTGCATATCCTTAAGAAGAGCTGTTTGGGAGTACTAGTATGCTCGGCGAGGTGTAGACTAGCTGATGGGTCCAGGGTGCACCTACGACCAGCTATTTGCGACAAGGCACGAAAGAAACAACAAGGTAAACCATGCCCGAACCGAATGTGCAACGGCGGCCGTTTAGAGGTCCAACCATGCCGGGGCCACTGTGGCTACCCAGTCACTCATTTCTGGAGACACACCGACCACGCTATATTCTTCCAAGCGAAGGGGGCGCACGACCACCCACGACCAGAAGCTAAGGGCGCCAGTGAAGTGCGGAGGTCTCTAGGTGCAGGGAGGAGGGTCAGGGGGCTGGCGTTGCTGCTCGCGAGGGAAGCGGCGATAGCGGACAAGATACTAACCGTTAAACCGGACAAACAAATGTCGCAGAAGATCTGCGCTCCCCACCAGCAGCCACCACCGCTCATACCCGACAATCAAAGAGGCCTAACATGCACCTGCGGACCTTTCGAATGCTCCTGCCGGTGGCGCACAGAGCATCCAACCGAAGCATACGCCGCGCCCGCGTGGTCGCCCATCGAGGCGCAAGCATACAACGCGTACGCACCCCCCGCGCCCCCCGCCCCCACGCTGCCCCACCAGCACTATGACCCCACCACCTTACCAGCGGACGATATCTTCCACCCCGAAGAGATATTCCAACTAGACCAACCCATCAGACTAGACTTCCCCATCGACGAAACCACGTTAGAATCACCACCCACCTTCGCCGACCTAAACGATAATTCGAGGCCCGACGACGCCTACTGGTTGGAGTGGCAGCGAGCGGCCGGCTCCGAATCCAGTGAAACACCATCCCCTGAACTGTTCGGCAACGGTTACCAGCAAGCTGAAGCGTATTGTGAACAGCAGAACTATCCCCCACAAATGTACTATCCTGAAGAAGCGCAATACTATCCAGCGGAAAGTACGAAAAACTCAGCAGTGATGGAAATGCAGGAGCAGAGGTACTATAGATACGGACAGGACTGCGGGCAGAATAGTATGGATATGCAAGCGTGGAATTACACTGACTGCGCTTTCCCGGCCAACGATGTGTCCGAATGCAAACAGTATTTCGACGTGCAACACCATCAAACAATGAACGCGTTCAGCGAGCTTTTATAA
Protein Sequence
MYPLWPLVWSFAHSTYNFYRNIINPSLGSVELSCKADASNAWLSACQNSILPLLPQSQRQWDEPLCKQVLEGLISTSSDMPDIARLTYCRLPESQATGSGITICQCRTSSRADMSEGPATPEWDINDAVVPRVSSFDTFSEWCDGHVRRVYPPGCEEARRHASGWAMRNTNNHNVHILKKSCLGVLVCSARCRLADGSRVHLRPAICDKARKKQQGKPCPNRMCNGGRLEVQPCRGHCGYPVTHFWRHTDHAIFFQAKGAHDHPRPEAKGASEVRRSLGAGRRVRGLALLLAREAAIADKILTVKPDKQMSQKICAPHQQPPPLIPDNQRGLTCTCGPFECSCRWRTEHPTEAYAAPAWSPIEAQAYNAYAPPAPPAPTLPHQHYDPTTLPADDIFHPEEIFQLDQPIRLDFPIDETTLESPPTFADLNDNSRPDDAYWLEWQRAAGSESSETPSPELFGNGYQQAEAYCEQQNYPPQMYYPEEAQYYPAESTKNSAVMEMQEQRYYRYGQDCGQNSMDMQAWNYTDCAFPANDVSECKQYFDVQHHQTMNAFSELL

Similar Transcription Factors

Sequence clustering based on sequence similarity using MMseqs2

100% Identity
iTF_00683093; iTF_00681436; iTF_01246593; iTF_00171522; iTF_01192386; iTF_01361346; iTF_00273379; iTF_00818915; iTF_01500868; iTF_00036389; iTF_01193382; iTF_00124935; iTF_00300935; iTF_01081351; iTF_00006060; iTF_00278878; iTF_00039560; iTF_00122120; iTF_00373672; iTF_01094664; iTF_01208968; iTF_00150249; iTF_00176873; iTF_00928425; iTF_00953289; iTF_00959937; iTF_00958473; iTF_00959181; iTF_00842417; iTF_01033489; iTF_00824475; iTF_00954173; iTF_01034303; iTF_00041546; iTF_01071402; iTF_01084010; iTF_01084902; iTF_00967819; iTF_00071180; iTF_00345580; iTF_00673260; iTF_01025740; iTF_01362861; iTF_01424813; iTF_00040558; iTF_00123118; iTF_00172494; iTF_00341102; iTF_00026607; iTF_00027525; iTF_00887983; iTF_01061647; iTF_01281071; iTF_01436783; iTF_00113904; iTF_01312011; iTF_00113108; iTF_00112281; iTF_00448784; iTF_00449890; iTF_01338254; iTF_00425137; iTF_00622576; iTF_00745392; iTF_01279952; iTF_00355889; iTF_00354855; iTF_00751793; iTF_00408165; iTF_00757905; iTF_01150993; iTF_00063346; iTF_01010135; iTF_00185906; iTF_00363794; iTF_00037542; iTF_00667124; iTF_00844082; iTF_00237235; iTF_00445875; iTF_00818131; iTF_00830931; iTF_01030900; iTF_01440756; iTF_00177845; iTF_00427861; iTF_01429702; iTF_01124373; iTF_01125212; iTF_00905878; iTF_01019990; iTF_01377238; iTF_00017111; iTF_00926359; iTF_00935447; iTF_01317047; iTF_01316092; iTF_00038456; iTF_00783192; iTF_00784163; iTF_00785786; iTF_00836280; iTF_00837235; iTF_00834394; iTF_01028984; iTF_00771641; iTF_00826585; iTF_01027065; iTF_00017946; iTF_00075226; iTF_00186906; iTF_00186905; iTF_00277146; iTF_00711578; iTF_01064398; iTF_01062520; iTF_00859226; iTF_00290434; iTF_00787389; iTF_01358653; iTF_01028026; iTF_01487482; iTF_00147067; iTF_01063515; iTF_00347292; iTF_00907644; iTF_00960700; iTF_01526780; iTF_00621789; iTF_00042399; iTF_01073375; iTF_01092831; iTF_01180095; iTF_01179272; iTF_01285311; iTF_00120256; iTF_00809824; iTF_01503616; iTF_01525696; iTF_01331196;
90% Identity
iTF_01334528;
80% Identity
-