Basic Information

Transcription Factor Domain

TF Family: HSF
Domain: HSF_DNA-bind domain
PFAM: PF00447
TF Group: Helix-turn-helix
Description: Heat shock factor (HSF) is a transcriptional activator of heat shock genes [1, 4]: it binds specifically to heat shock promoter elements, which are palindromic sequences rich with repetitive purine and pyrimidine motifs [1]. Under normal conditions, HSF is a homo-trimeric cytoplasmic protein, but heat shock activation results in relocalisation to the nucleus [2]. Each HSF monomer contains one C-terminal and three N-terminal leucine zipper repeats [3]. Point mutations in these regions result in disruption of cellular localisation, rendering the protein constitutively nuclear [2]. Two sequences flanking the N-terminal zippers fit the consensus of a bi- partite nuclear localisation signal (NLS). Interaction between the N- and C-terminal zippers may result in a structure that masks the NLS sequences: following activation of HSF, these may then be unmasked, resulting in relocalisation of the protein to the nucleus [3]. The DNA-binding component of HSF lies to the N terminus of the first NLS region, and is referred to as the HSF domain.
Hmmscan Out: # of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc

1 3 1.9e-21 3.8e-17 64.4 0.0 1 100 89 189 89 189 0.92

2 3 2 4.1e+04 -3.2 0.0 79 93 235 251 221 258 0.55

3 3 0.74 1.5e+04 -1.8 0.0 74 97 308 331 298 333 0.73

#	of	c-Evalue	i-Evalue	score	hmm coord from	hmm coord to	ali coord from	ali coord to	env coord from	env coord to	acc
1	3	1.9e-21	3.8e-17	64.4	1	100	89	189	89	189	0.92
2	3	2	4.1e+04	-3.2	79	93	235	251	221	258	0.55
3	3	0.74	1.5e+04	-1.8	74	97	308	331	298	333	0.73

Coding Sequence: ATGGCAATTGAATCTAAGACGATTGAACTGATGAGCATGCTTTTAGAATTATTCTACTTCTCGCTGGCGTTGAAGTTGAACTGTGATACAATTCTTACTTCGGATCTTCCACAAGAAACTTCTACACCCCAATCGCAGCCGTCAGAATTCGAATCAACGAAAAATAACGTGACTCCGGTGGCGCTGCCATTGCAAGCTTCCGAGCCTCTGGAAACGGTGTCCGATGTAGCTTCTTCGGAGGACAGCGCGATTCCGACTCTGCGTTTTcctcaaaagctctggcaaaTTGTGAATGAATGCAAAACTGGTGCGATAACGCGGGGACCGGGTGGTTATACTGTCTTTGTCGATGAACGTAAATTCACCGCAGAGTTTTTGACCTGCGAAAATCCTTTATTCAAGACTCGTATCTTTACGAGCTTTGTTCGGCAGCTGAACCTCTATGGATTTCGCAAGGTGACAACTTCGCGAGGCCGACAGCACAACGGCACTGGACCAAGCTGCGAATTTTTACACGAACATTTTCGTTTTGGAAGGCTCGATCTGCTATCCCGTGTCTGTCGGAAAGCCTGCGTAAAGAGGAATTATCCTAGACAGAGGAAAAGATGGGTCAAGGTGGAGCCAGATATTGATCCCATCACCGGTTTGACGAAGCTTGAAAAGTGTCAGATTGCACTCACAGAAGCTTTGGAGCAAGCCACTAGACAATATAGAGAAGAACAGCTCAAGTTTCAAAGGCAAAGTATAAGGATCTTGTCAAGGTTGAGGAAAAATCACTCGTCAAAAGTATTTGAAGCAGAGCCAAATATAAAAGTAGTTATTGGAGATATGATACAGATTGTTGATGTAGGGAATTTACCTATTATTGATGCTTCTATAATACCTGTCATCGCCAATGCATCTGGCTGTGAATATCAAAGACAATGGGAACAGTTATTGACCTTTACAACTGAAAATTTCGCTATTTCAGCGCCTcaaaaatgtgaaaagttgGAGTCTCCATGA
Protein Sequence: MAIESKTIELMSMLLELFYFSLALKLNCDTILTSDLPQETSTPQSQPSEFESTKNNVTPVALPLQASEPLETVSDVASSEDSAIPTLRFPQKLWQIVNECKTGAITRGPGGYTVFVDERKFTAEFLTCENPLFKTRIFTSFVRQLNLYGFRKVTTSRGRQHNGTGPSCEFLHEHFRFGRLDLLSRVCRKACVKRNYPRQRKRWVKVEPDIDPITGLTKLEKCQIALTEALEQATRQYREEQLKFQRQSIRILSRLRKNHSSKVFEAEPNIKVVIGDMIQIVDVGNLPIIDASIIPVIANASGCEYQRQWEQLLTFTTENFAISAPQKCEKLESP

Sequence clustering based on sequence similarity using MMseqs2