Basic Information

Gene Symbol
-
Assembly
GCA_947578815.1
Location
OX388221.1:465902-467023[+]

Transcription Factor Domain

TF Family
GCM
Domain
GCM domain
PFAM
PF03615
TF Group
Beta-Scaffold Factors
Description
GCM transcription factors are a family of proteins which contain a GCM motif. The GCM motif is a domain that has been identified in proteins belonging to a family of transcriptional regulators involved in fundamental developmental processes which comprise Drosophila melanogaster GCM and its mammalian homologues [PMID: 8962155, PMID: 9114061, PMID: 9580683, PMID: 10671510]. IN GCM transcription factors the N-terminal moiety contains a DNA-binding domain of 150 residues. Sequence conservation is highest in this GCM domain. In contrast, the C-terminal moiety contains one or two transactivating regions and is only poorly conserved.The GCM motif has been shown to be a DNA binding domain that recognises preferentially the nonpalindromic octamer 5'-ATGCGGGT-3' [PMID: 8962155, PMID: 9114061, PMID: 9580683]. The GCM motif contains many conserved basic amino acid residues, seven cysteine residues, and four histidine residues [PMID: 8962155]. The conserved cysteines are involved in shaping the overall conformation of the domain, in the process of DNA binding and in the redox regulation of DNA binding [PMID: 9580683]. The GCM domain as a new class of Zn-containing DNA-binding domain with no similarity to any other DNA-binding domain [PMID: 12682016]. The GCM domain consists of a large and a small domain tethered together by one of the two Zn ions present in the structure. The large and the small domains comprise five- and three-stranded beta-sheets, respectively, with three small helical segments packed against the same side of the two beta-sheets. The GCM domain exercises a novel mode of sequence-specific DNA recognition, where the five-stranded beta-pleated sheet inserts into the major groove of the DNA. Residues protruding from the edge strand of the beta-pleated sheet and the following loop and strand contact the bases and backbone of both DNA strands, providing specificity for its DNA target site.
Hmmscan Out
# of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc
1 7 0.0023 41 5.0 0.1 62 101 16 54 10 62 0.85
2 7 0.0025 45 4.9 0.1 62 101 68 106 60 114 0.84
3 7 0.0019 34 5.3 0.1 62 101 120 158 105 166 0.85
4 7 0.0027 47 4.8 0.1 62 101 172 210 166 219 0.84
5 7 0.0052 91 3.9 0.0 62 101 224 262 215 271 0.84
6 7 0.0025 45 4.9 0.1 62 100 276 313 267 321 0.84
7 7 0.0032 55 4.6 0.1 61 96 327 361 317 367 0.85

Sequence Information

Coding Sequence
ATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACACTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACACTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACACTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCGGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACACTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACTCTACAGTTCGCATGTCCGTGTCCGTCCCGCTGTCCGGCAGTCCTCCTCTACACTACAGTTCGCATTTCTCAACCGATTCTCGGGCAATTTTGA
Protein Sequence
MSVSVPLSGSPPLLYSSHVRVRPAVRQSSSTLQFACPCPSRCPAVLLYTTVRMSVSVPLSGSPPLLYSSHVRVRPAVRQSSSTLQFACPCPSRCPAVLLYSTVRMSVSVPLSGSPPLLYSSHVRVRPAVRQSSSTLQFACPCPSRCPAVLLYTTVRMSVSVPLSGSPPLLYSSHVRVRPAVRQSSSTLQFACPCPSRCPAVLLYSTVRMSVSVPLSGSPPLLYSSHVGVRPAVRQSSSTLQFACPCPSRCPAVLLYSTVRMSVSVPLSGSPPLLYSSHVRVRPAVRQSSSTLQFACPCPSRCPAVLLYSTVRMSVSVPLSGSPPLHYSSHVRVRPAVRQSSSTLQFACPCPSRCPAVLLYTTVRISQPILGQF

Similar Transcription Factors

Sequence clustering based on sequence similarity using MMseqs2

100% Identity
-
90% Identity
-
80% Identity
-