Basic Information

Transcription Factor Domain

TF Family: HSF
Domain: HSF_DNA-bind domain
PFAM: PF00447
TF Group: Helix-turn-helix
Description: Heat shock factor (HSF) is a transcriptional activator of heat shock genes [1, 4]: it binds specifically to heat shock promoter elements, which are palindromic sequences rich with repetitive purine and pyrimidine motifs [1]. Under normal conditions, HSF is a homo-trimeric cytoplasmic protein, but heat shock activation results in relocalisation to the nucleus [2]. Each HSF monomer contains one C-terminal and three N-terminal leucine zipper repeats [3]. Point mutations in these regions result in disruption of cellular localisation, rendering the protein constitutively nuclear [2]. Two sequences flanking the N-terminal zippers fit the consensus of a bi- partite nuclear localisation signal (NLS). Interaction between the N- and C-terminal zippers may result in a structure that masks the NLS sequences: following activation of HSF, these may then be unmasked, resulting in relocalisation of the protein to the nucleus [3]. The DNA-binding component of HSF lies to the N terminus of the first NLS region, and is referred to as the HSF domain.
Hmmscan Out: # of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc

1 3 2.4e-29 5.3e-25 89.7 0.7 1 100 14 116 14 116 0.92

2 3 0.23 5e+03 -0.1 0.1 7 88 162 174 121 199 0.63

3 3 0.29 6.3e+03 -0.4 0.1 7 39 162 194 153 233 0.69

#	of	c-Evalue	i-Evalue	score	bias	hmm coord from	hmm coord to	ali coord from	ali coord to	env coord from	env coord to	acc
1	3	2.4e-29	5.3e-25	89.7	0.7	1	100	14	116	14	116	0.92
2	3	0.23	5e+03	-0.1	0.1	7	88	162	174	121	199	0.63
3	3	0.29	6.3e+03	-0.4	0.1	7	39	162	194	153	233	0.69

Coding Sequence: ATGACAACTTATAATGAGTATACTATACAGGCGGTGAAGTTTCCTCAAAAACTGTGGAAAATAGTCAACGATTGCGAGACAGGTGCTATCCAGTGGAGCCATAGTGGAAATTCAATTCTTCTCGAATACAATAAGTttcaaaaagaatatttgacCCTAAACACTTCCATTTTTAAGACCACTAACATCACCAGTTTTATAAGACAGCTTAATTTATATGGTTTTCGAAAAGTAACGTCTCATCGTCGTGATCCTATTTGTAATTCACATAACCCTAATATGCACGAGTTTTTACATGAATACTTTCGGATTGATCGCCCCGATCTCTTGAAGAAAGTTTGTCGCAAAATAAATACTACAACAAAATCGATTCAGAAACAGAATCATTCTTTCAAGAATCCAAATAAATGTGACATACCGAGCTCAGAAAGAGGCATGACAAAATTACAAATATGTCAGaacGCCTTGAAAAAAGCTCTGGAACAAGCTTTGCATCAATATAAACAAGATAAATTTATCAGATTAATGGAGGATATCGCAAATTTTTCCCCAGAGGATGCAGAAAGTTCTCAGGATACAATCTTAACACTAATTGAAATGAAATCCCCCGAAGACGTAGAAGAAGAAAGTTCCAGTGAGTTTGGAACCCAAGAAAGTAACGACGACGTGATTTATAAATTGTCTGGTTACAAGTTTCTTGTTCCAATCAATGAATCTACTCAACGATTTAATCCTTATTTTGTCAATCGAAATGAATATACTCTAAGCGACGAGATTTGA
Protein Sequence: MTTYNEYTIQAVKFPQKLWKIVNDCETGAIQWSHSGNSILLEYNKFQKEYLTLNTSIFKTTNITSFIRQLNLYGFRKVTSHRRDPICNSHNPNMHEFLHEYFRIDRPDLLKKVCRKINTTTKSIQKQNHSFKNPNKCDIPSSERGMTKLQICQNALKKALEQALHQYKQDKFIRLMEDIANFSPEDAESSQDTILTLIEMKSPEDVEEESSSEFGTQESNDDVIYKLSGYKFLVPINESTQRFNPYFVNRNEYTLSDEI

Sequence clustering based on sequence similarity using MMseqs2