Basic Information

Transcription Factor Domain

TF Family: HSF
Domain: HSF_DNA-bind domain
PFAM: PF00447
TF Group: Helix-turn-helix
Description: Heat shock factor (HSF) is a transcriptional activator of heat shock genes [1, 4]: it binds specifically to heat shock promoter elements, which are palindromic sequences rich with repetitive purine and pyrimidine motifs [1]. Under normal conditions, HSF is a homo-trimeric cytoplasmic protein, but heat shock activation results in relocalisation to the nucleus [2]. Each HSF monomer contains one C-terminal and three N-terminal leucine zipper repeats [3]. Point mutations in these regions result in disruption of cellular localisation, rendering the protein constitutively nuclear [2]. Two sequences flanking the N-terminal zippers fit the consensus of a bi- partite nuclear localisation signal (NLS). Interaction between the N- and C-terminal zippers may result in a structure that masks the NLS sequences: following activation of HSF, these may then be unmasked, resulting in relocalisation of the protein to the nucleus [3]. The DNA-binding component of HSF lies to the N terminus of the first NLS region, and is referred to as the HSF domain.
Hmmscan Out: # of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc

1 3 4.7e-18 2e-14 53.8 2.2 1 99 20 115 20 116 0.85

2 3 0.41 1.8e+03 -0.5 0.0 14 36 220 242 209 258 0.74

3 3 1.3 5.6e+03 -2.1 0.1 70 92 309 331 279 335 0.49

#	of	c-Evalue	i-Evalue	score	bias	hmm coord from	hmm coord to	ali coord from	ali coord to	env coord from	env coord to	acc
1	3	4.7e-18	2e-14	53.8	2.2	1	99	20	115	20	116	0.85
2	3	0.41	1.8e+03	-0.5	0.0	14	36	220	242	209	258	0.74
3	3	1.3	5.6e+03	-2.1	0.1	70	92	309	331	279	335	0.49

Coding Sequence: ATGGATACATCTCATAGAATAAAACTAAAAAGCTTACGTTACATTCGTAGTATGAGATTCCCCATAAAACTTTATAGTGCTTGTGAAAGCCAAAATATATTATCAATAAATTGGAGTTTAAATGAAGAAACATCACTTGTAATTGATTTAAAAGAACTTGAAAGTTATTTAAAAAAAGTAACATCAATATTTCTTACAAATAATATATCACAATTTCTTACTGAATTAAAAATATATGATTTCGAAGAAATAAATCAGAATAATTCAGATTCTGAACACATAAAAAAATTTTATCATCAATATTTCCTTCGTGGTAAGCCAGAATTATTAAAAAATATTATGCAAAATGAAAATTTTAATGAATTATTAAAATATCGAGAAATTAAAAAATCACATCGTTTATTAAAATTTACATTACAAGGGAAATATCTAATTGATAAAATACGTGGTGATTTATGTTTAAATAATGAATCTACACAATTACTTGCATTTCCATCTAAACGTGTATTATTACCTGGAGATACTGTATCAGGTTTCGAAGAGAATTCAGATTATGCTGGCTTTTATGGGCATTATGACATCGATGCAGAAAAAGTTAAGAAATTTTATGATGAATTTTTACCAACAATATCAACAAATCCAGTAACATATGGTCAATCACAACAAGCAGTACATGATGATGAAGATGGTTCTCATGTAGTAATTCATCAAAATAAGTTCATTAATGATGAATCAGCAGTTCCTGCTCCAAATTATGAGCACAGTTATATTTTACAAGGTTATAATGAAGAACAAATTTTACCTGATGATGAAATAACAATGGTTTCGACCAATGAGGATAACCTTAATATGGATAATTTTTTGGTTTTTAATAATAAGGCAACAGTAGAGGATCAAGCAATGAATTCTGTAGAAGACACCGAAATGAAATCAACAGAAGACAATGAAAATTCAGAATTAAATGATGATAATTTTTTATCTCAAAGCATGTCACTATTAGTGAATAATGATTAA
Protein Sequence: MDTSHRIKLKSLRYIRSMRFPIKLYSACESQNILSINWSLNEETSLVIDLKELESYLKKVTSIFLTNNISQFLTELKIYDFEEINQNNSDSEHIKKFYHQYFLRGKPELLKNIMQNENFNELLKYREIKKSHRLLKFTLQGKYLIDKIRGDLCLNNESTQLLAFPSKRVLLPGDTVSGFEENSDYAGFYGHYDIDAEKVKKFYDEFLPTISTNPVTYGQSQQAVHDDEDGSHVVIHQNKFINDESAVPAPNYEHSYILQGYNEEQILPDDEITMVSTNEDNLNMDNFLVFNNKATVEDQAMNSVEDTEMKSTEDNENSELNDDNFLSQSMSLLVNND

Sequence clustering based on sequence similarity using MMseqs2