g3451.t1


Basic Information
Transcription Factor Domain
Sequence Information
Similer Transcription Factors

Basic Information

Insect
Amphipyra tragopoginis
Gene Symbol
gcm
Assembly
GCA_905220435.1
Location
HG992000.1:17686530-17688946[-]

Transcription Factor Domain

TF Family
GCM
Domain
GCM domain
PFAM
PF03615
TF Group
Beta-Scaffold Factors
Description
GCM transcription factors are a family of proteins which contain a GCM motif. The GCM motif is a domain that has been identified in proteins belonging to a family of transcriptional regulators involved in fundamental developmental processes which comprise Drosophila melanogaster GCM and its mammalian homologues [PMID: 8962155, PMID: 9114061, PMID: 9580683, PMID: 10671510]. IN GCM transcription factors the N-terminal moiety contains a DNA-binding domain of 150 residues. Sequence conservation is highest in this GCM domain. In contrast, the C-terminal moiety contains one or two transactivating regions and is only poorly conserved.The GCM motif has been shown to be a DNA binding domain that recognises preferentially the nonpalindromic octamer 5'-ATGCGGGT-3' [PMID: 8962155, PMID: 9114061, PMID: 9580683]. The GCM motif contains many conserved basic amino acid residues, seven cysteine residues, and four histidine residues [PMID: 8962155]. The conserved cysteines are involved in shaping the overall conformation of the domain, in the process of DNA binding and in the redox regulation of DNA binding [PMID: 9580683]. The GCM domain as a new class of Zn-containing DNA-binding domain with no similarity to any other DNA-binding domain [PMID: 12682016]. The GCM domain consists of a large and a small domain tethered together by one of the two Zn ions present in the structure. The large and the small domains comprise five- and three-stranded beta-sheets, respectively, with three small helical segments packed against the same side of the two beta-sheets. The GCM domain exercises a novel mode of sequence-specific DNA recognition, where the five-stranded beta-pleated sheet inserts into the major groove of the DNA. Residues protruding from the edge strand of the beta-pleated sheet and the following loop and strand contact the bases and backbone of both DNA strands, providing specificity for its DNA target site.
Hmmscan Out
# of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc
1 1 1e-76 8.9e-73 241.7 2.9 1 140 22 162 22 162 0.98

Sequence Information

Coding Sequence
ATGTCGGAGGGCCAAACACCGGACTgggacataaacgatgcagtcGTGCCGCGCGTAGCCGCGTTCGACACGTTCGGCGAGTGGTGCGACGGACATGTGCGGCGCGTGTACCCGCCGGGCTGCGAGGAGGCGCGCCGCCACGCCTCGGGCTGGGCCATGCGCAACACTAACAACCACAACGTGCACATACTGAAGAAGAGCTGCCTTGGAGTACTGGTGTGCTCACAACGCTGTCGCTTGCCTGACGGCTCGAGAGTACATCTTCGGCCCGCCATATGTGATAAAGCGAGGAAGAAGCAGCAAGGCAAACCATGTCCAAACAGACTATGCAACGGCGGTCGTTTAGAAGTCCTACCATGTAGAGGACACTGCGGCTACCCCGTCACTCACTTTTGGCGACACACGGAACACGCCATCTTCTTCCAAGCGAAAGGCGCCCACGACCATCCAAGACCTGAAGCTAAGGGAGCGAGCGAAGTGCGGCGGTCCCTGGGCGCCGGCAGAAGAGTAAGAGGCTTAGCGCTACTGCTGGCGAGAGAAGCAGCCATAGCAGACAAGATATTGAAACCAGACAAGCAAATGACCCAGAAAATGTGCAATCCACATGCACAACCCCCTCCACTAATACCAGATAACCAAAGAGTTCAACTAACATGCACCTGTGGGCCTTTCGAGTGCTCATGTCGGTGGCGTCCCGAACAGACACCGGAGAACTACGCAACAGCGGCGTGGTCCCCACAAGAGCAACAGTACCAGACGTACGCACAGCCCGTGCCCCCCGGACCCTCACTGCAGCCGCAGCAACACTACGACCCCACAGCACTACCGGCCGACGACATCTTCCACCCCGAGGAAATCTTCCAGCTCGACCAACCTATCAGACTAGACTTCCCATTAGAAGAGCATACCTTAGAATCACCACCCACCTTCGCAGATCTCAATAGCGAAAACTCTAGGCCCGAAGAAGCGTACTGGTTGGACTGGCAACGAGCTGCAGGCGGCTCAGAGTCCAGTGAAACCCCTTCACCTGAACTGTTCGGTGGTTACCAGCCGACTGAAGCCTATTGCGAACAACAAACATACATCCCGCAAGCGTATTACCCTGAAGAGGCGCAATACTATCCCGCTGAAAGCAGAACCTCCCCTATAATGGACATGCAGGAACAAAGGTTCTATAGGTACGGCCAGGATTGTACTCAAACTAACGTAGATATGCAGACTTGGAACTACACGGACTGCGCTTTTACCTCCAATGACATGTCAGAATGTAAACAGTACTTCGATGTTCAGCATCAACAAGCCGTGAACGCCTTCAGTGGGCTCTTATAA
Protein Sequence
MSEGQTPDWDINDAVVPRVAAFDTFGEWCDGHVRRVYPPGCEEARRHASGWAMRNTNNHNVHILKKSCLGVLVCSQRCRLPDGSRVHLRPAICDKARKKQQGKPCPNRLCNGGRLEVLPCRGHCGYPVTHFWRHTEHAIFFQAKGAHDHPRPEAKGASEVRRSLGAGRRVRGLALLLAREAAIADKILKPDKQMTQKMCNPHAQPPPLIPDNQRVQLTCTCGPFECSCRWRPEQTPENYATAAWSPQEQQYQTYAQPVPPGPSLQPQQHYDPTALPADDIFHPEEIFQLDQPIRLDFPLEEHTLESPPTFADLNSENSRPEEAYWLDWQRAAGGSESSETPSPELFGGYQPTEAYCEQQTYIPQAYYPEEAQYYPAESRTSPIMDMQEQRFYRYGQDCTQTNVDMQTWNYTDCAFTSNDMSECKQYFDVQHQQAVNAFSGLL*

Similar Transcription Factors

Sequence clustering based on sequence similarity using MMseqs2

100% Identity
iTF_01173042;
90% Identity
iTF_00171522; iTF_01341986; iTF_00924358; iTF_00667124; iTF_00237235; iTF_01259848; iTF_00041546; iTF_01439660; iTF_01538508; iTF_01221261; iTF_01424813; iTF_00040558; iTF_00172494; iTF_01246593; iTF_01192386; iTF_00273379; iTF_00808869; iTF_00037542; iTF_00274215; iTF_00951560; iTF_01094664; iTF_00176873; iTF_00071180; iTF_00299916; iTF_01025740; iTF_01093805; iTF_01338254; iTF_00745392; iTF_00973488; iTF_00425137; iTF_00757905; iTF_00363794; iTF_00952472; iTF_00771641; iTF_01092831; iTF_00120256; iTF_00809824; iTF_00124001; iTF_00122120; iTF_00373672; iTF_00121163; iTF_01339725; iTF_00123118; iTF_01028984; iTF_00887983; iTF_00711578; iTF_01084902; iTF_00300935; iTF_00301864; iTF_00928425; iTF_01084010; iTF_01064398; iTF_01061647; iTF_01062520; iTF_01028026; iTF_00907644; iTF_00851485; iTF_00043247; iTF_00039560; iTF_00448784; iTF_00726052; iTF_00449890; iTF_00622576; iTF_01030900; iTF_00147067; iTF_00042399; iTF_01173042; iTF_00445875; iTF_00446882; iTF_01533703; iTF_00444873; iTF_00685197; iTF_01531731; iTF_01532793; iTF_01534556; iTF_00830931; iTF_01440756; iTF_00185077; iTF_00185906; iTF_01377238; iTF_00000951; iTF_00326852; iTF_00000085; iTF_00017111; iTF_00783192; iTF_00784163; iTF_00784966; iTF_00785786; iTF_00383385; iTF_00049665; iTF_00017946; iTF_01487482; iTF_01063515; iTF_01285311; iTF_01525696; iTF_01526780; iTF_00906825; iTF_00036389; iTF_00905878; iTF_00374935; iTF_00038456;
80% Identity
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