Basic Information

Transcription Factor Domain

TF Family: HSF
Domain: HSF_DNA-bind domain
PFAM: PF00447
TF Group: Helix-turn-helix
Description: Heat shock factor (HSF) is a transcriptional activator of heat shock genes [1, 4]: it binds specifically to heat shock promoter elements, which are palindromic sequences rich with repetitive purine and pyrimidine motifs [1]. Under normal conditions, HSF is a homo-trimeric cytoplasmic protein, but heat shock activation results in relocalisation to the nucleus [2]. Each HSF monomer contains one C-terminal and three N-terminal leucine zipper repeats [3]. Point mutations in these regions result in disruption of cellular localisation, rendering the protein constitutively nuclear [2]. Two sequences flanking the N-terminal zippers fit the consensus of a bi- partite nuclear localisation signal (NLS). Interaction between the N- and C-terminal zippers may result in a structure that masks the NLS sequences: following activation of HSF, these may then be unmasked, resulting in relocalisation of the protein to the nucleus [3]. The DNA-binding component of HSF lies to the N terminus of the first NLS region, and is referred to as the HSF domain.
Hmmscan Out: # of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc

1 2 2e-37 1.2e-33 116.0 0.1 1 100 14 113 14 113 0.97

2 2 2.1 1.2e+04 -2.8 0.0 16 41 119 142 116 152 0.66

#	of	c-Evalue	i-Evalue	score	bias	hmm coord from	hmm coord to	ali coord from	ali coord to	env coord from	env coord to	acc
1	2	2e-37	1.2e-33	116.0	0.1	1	100	14	113	14	113	0.97
2	2	2.1	1.2e+04	-2.8	0.0	16	41	119	142	116	152	0.66

Coding Sequence: ATGCGGTCAGTTGTGGAAGTCGGAGCTACTGTCCCTGCTTTTTTGGGAAAATTGTGGAAATTGGTGAACGATTCTGAGACTAATCACTTGATATCATGGAGTCCTGGAGGGAAAACATTTGTTATAAAAAATCAGGCAGATTTTGCAAGGGATCTATTACCCTTGTACTACAAACACAACAACATGGCAAGCTTTATCAGGCAGCTCAACATGTATGGCTTTCATAAAATCACCTCTGTCGAGAATGGCGGCCTGAGGTATGAGAAGGACGAGATTGAATTTTCACATCCATGTTTCATGAAGGGCCATGCTTACCTCCTAGAACACATCAAAAGAAAAATAGCCAATCCAAAGTCCATAGTCACCAGTAATGAAAGCGGAGAAAAGATACTTTTGAAGCCAGAACTTATGAACAAAGTTTTATCTGATGTGAAATCAATGAAAGGCAAACAAGAAAGTCTTGATGCAAAGTTTAGTGCTATGAAGCAAGAAAATGAAGCTCTATGGAGGGAGGTGGCCATCCTAAGGCAGAAACATATCAAGCAACAGCAGATTGTCAACAATCTTATTCAATTCTTGATGTCTCTGGTACAACCAGGAAGAGCAGGCAACCCTAACGGTAACAATGTAGGAGTTAAGAGGCCGTACCAATTGATGATCAATAATGCGGCGCATAACTCTGGCGATGGCACATATCCTGGACGATTCAAAAATCTGAAACTTGACAAGGACACGTTACTGGAAGATATGAATGAAGATAACTTGGTAAGAAATTATTTCAATGAAGGTTAG
Protein Sequence: MRSVVEVGATVPAFLGKLWKLVNDSETNHLISWSPGGKTFVIKNQADFARDLLPLYYKHNNMASFIRQLNMYGFHKITSVENGGLRYEKDEIEFSHPCFMKGHAYLLEHIKRKIANPKSIVTSNESGEKILLKPELMNKVLSDVKSMKGKQESLDAKFSAMKQENEALWREVAILRQKHIKQQQIVNNLIQFLMSLVQPGRAGNPNGNNVGVKRPYQLMINNAAHNSGDGTYPGRFKNLKLDKDTLLEDMNEDNLVRNYFNEG

Sequence clustering based on sequence similarity using MMseqs2