Aful041411.1
Basic Information
- Insect
- Agonum fuliginosum
- Gene Symbol
- -
- Assembly
- GCA_947534325.1
- Location
- OX384043.1:2696586-2698875[-]
Transcription Factor Domain
- TF Family
- GCM
- Domain
- GCM domain
- PFAM
- PF03615
- TF Group
- Beta-Scaffold Factors
- Description
- GCM transcription factors are a family of proteins which contain a GCM motif. The GCM motif is a domain that has been identified in proteins belonging to a family of transcriptional regulators involved in fundamental developmental processes which comprise Drosophila melanogaster GCM and its mammalian homologues [PMID: 8962155, PMID: 9114061, PMID: 9580683, PMID: 10671510]. IN GCM transcription factors the N-terminal moiety contains a DNA-binding domain of 150 residues. Sequence conservation is highest in this GCM domain. In contrast, the C-terminal moiety contains one or two transactivating regions and is only poorly conserved.The GCM motif has been shown to be a DNA binding domain that recognises preferentially the nonpalindromic octamer 5'-ATGCGGGT-3' [PMID: 8962155, PMID: 9114061, PMID: 9580683]. The GCM motif contains many conserved basic amino acid residues, seven cysteine residues, and four histidine residues [PMID: 8962155]. The conserved cysteines are involved in shaping the overall conformation of the domain, in the process of DNA binding and in the redox regulation of DNA binding [PMID: 9580683]. The GCM domain as a new class of Zn-containing DNA-binding domain with no similarity to any other DNA-binding domain [PMID: 12682016]. The GCM domain consists of a large and a small domain tethered together by one of the two Zn ions present in the structure. The large and the small domains comprise five- and three-stranded beta-sheets, respectively, with three small helical segments packed against the same side of the two beta-sheets. The GCM domain exercises a novel mode of sequence-specific DNA recognition, where the five-stranded beta-pleated sheet inserts into the major groove of the DNA. Residues protruding from the edge strand of the beta-pleated sheet and the following loop and strand contact the bases and backbone of both DNA strands, providing specificity for its DNA target site.
- Hmmscan Out
-
# of c-Evalue i-Evalue score bias hmm coord from hmm coord to ali coord from ali coord to env coord from env coord to acc 1 16 0.18 3.1e+03 -0.5 0.0 26 49 6 29 2 41 0.78 2 16 0.0037 63 5.0 0.1 21 56 41 76 31 88 0.79 3 16 0.0037 64 4.9 0.1 20 56 100 136 89 145 0.79 4 16 0.024 4.2e+02 2.3 0.1 25 50 145 170 134 181 0.80 5 16 0.014 2.3e+02 3.1 0.0 21 49 161 189 151 192 0.75 6 16 0.00056 9.7 7.6 0.1 21 56 181 216 170 228 0.78 7 16 0.0036 61 5.0 0.1 21 56 241 276 231 288 0.79 8 16 0.032 5.5e+02 1.9 0.0 25 49 285 309 276 318 0.83 9 16 0.004 68 4.9 0.1 23 56 303 336 291 347 0.75 10 16 0.011 1.9e+02 3.4 0.1 23 49 323 349 311 361 0.74 11 16 0.0037 64 4.9 0.1 21 56 361 396 351 406 0.79 12 16 0.025 4.2e+02 2.3 0.0 25 50 405 430 394 441 0.81 13 16 0.0038 65 4.9 0.1 23 56 423 456 411 468 0.74 14 16 0.01 1.7e+02 3.5 0.0 23 49 443 469 430 482 0.73 15 16 0.055 9.5e+02 1.2 0.1 23 49 463 489 453 499 0.75 16 16 0.033 5.6e+02 1.9 0.0 22 49 482 509 471 528 0.72
Sequence Information
- Coding Sequence
- ATGGAATTAGAGCTCCACAAACTCGGTTTTGGTATTTGCTACACTAACAAAGATAACAAAACCGTACTAGAGCCCCACAAACTCGGTTTTGGTATTTACTACACTAACAAAGCTAACAAAACCGTACTACAGCTCCACAAACTCGGTTTTTGTTTTGACTACACTAACAAAGCTAACAAAACTGTACTAGAGCTCCACAAACTCGGTTTTGGTATTTGCTACACTAACAAAGCTAACAAAACCGTACTAGAGCCCCACAAACTCGGTTTTGGTATTTACTACACTAACAAAGCTAACAAAACCGTACTACAGCTCCACAAACTCGGTTTTTGTTTTGACTACACTAACAAAGCTAACAAAACTGTACTAGAGCTCCACAAACTCGGTTTTGGTATTTGCTACACTAACAAAGCTAACAAAACCGTACTAGAGCCCCACAAACTCGGTTTTGGTATTTACTACACTAACAAAGCTAACAAAACCGTACTAAAGCCCCACAAACTCGGTTTTGGTATTTACTACACTAACAAAGCTAACAAAACCGTACTAAAGCCCCACAAACTCGGTTTTGGTATTTACTACACTAACAAAGCTAACAAAACCGTACTAAAGCCCCACAAACTCGGTTTTGGTATTTGCTACACTAACAAAGCTAACAAAACCGTACTAAAGCCCCACAAACTCGGTTTTGGTATTTACTACACTAACAAAGCTAACAAAACCGTACTACAGCCCCACAAACTCGGTTTTTGTTTTGACTACACTAACAAAGCTAACAAAACTGTACTAGAGCTCCACAAACTCGGTTTTGGTATTTGCTACACTAACAAAGCTAACAAAACCGTACTAGAGCCCCACAAACTCGGTTTTGGTATTTACTACACTAACAAAGCTAACAAAACCGTACTAAAGCCCCACAAACTCGGTTTTGGTATTTACTACACTGACAAAGCTAACAAAACCGTACTAAAGCCCCACAAACTCGGTTTTGGTATTTGCTACACTAACAAAGCTAACAAAACCGTACTAAAGCCCCACAAACTCGGTTTTGGTATTTACTACACTAACAAAGCTAACAAAACCGTACTACAGCCCCACAAACTCGGTTTTTGTTTTGACTACACTAACAAAGCTAACAAAACTGTACTAGAGCTCCACAAACTCGGTTTTGGTATTTGCTACACTAACAAAGCTAACAAAACCGTACTAGAGCCCCACAAACTCGGTTTTGGTATTTACTACACTAACAAAGCTAACAAAACCGTACTAAAGCCCCACAAACTCGGTTTTGGTATTTACTACACTGACAAAGCTAACAAAACCGTACTAAAGCCCCACAAACTCGGTTTTGGTATTTGCTACACTAACAAAGCTAACAAAACCGTACTAAAGCCCCACAAACTCGGTTTTGGTATTTACTACACTAACAAAGCTAACAAAACCGTACTACAGCCCCACAAACTCGGTTTTTGTTTTGACTACACTAACAAAGCTAACAAAACCGTACTAGAGCCCCACAAACTCGGTTTTGGTATTTACTACACTAACAAAGCTAACAAAACCGTACTAGAGCCCCTCAAACTCGGTTTTGGTATTTACTACACTAACAAAACCGTACTATAG
- Protein Sequence
- MELELHKLGFGICYTNKDNKTVLEPHKLGFGIYYTNKANKTVLQLHKLGFCFDYTNKANKTVLELHKLGFGICYTNKANKTVLEPHKLGFGIYYTNKANKTVLQLHKLGFCFDYTNKANKTVLELHKLGFGICYTNKANKTVLEPHKLGFGIYYTNKANKTVLKPHKLGFGIYYTNKANKTVLKPHKLGFGIYYTNKANKTVLKPHKLGFGICYTNKANKTVLKPHKLGFGIYYTNKANKTVLQPHKLGFCFDYTNKANKTVLELHKLGFGICYTNKANKTVLEPHKLGFGIYYTNKANKTVLKPHKLGFGIYYTDKANKTVLKPHKLGFGICYTNKANKTVLKPHKLGFGIYYTNKANKTVLQPHKLGFCFDYTNKANKTVLELHKLGFGICYTNKANKTVLEPHKLGFGIYYTNKANKTVLKPHKLGFGIYYTDKANKTVLKPHKLGFGICYTNKANKTVLKPHKLGFGIYYTNKANKTVLQPHKLGFCFDYTNKANKTVLEPHKLGFGIYYTNKANKTVLEPLKLGFGIYYTNKTVL
Similar Transcription Factors
Sequence clustering based on sequence similarity using MMseqs2
- 100% Identity
- -
- 90% Identity
- -
- 80% Identity
- -